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Published on: September 14, 2019
Identification of novel immunogenic proteins against Streptococcus parauberis in a zebrafish model by reverse
Young Su Kim1, Nam-Kyung Yoon2, Nadia Karisa3
1Department of Chemical and Biomolecular Engineering, KAIST, Daejeon, 305-701, Republic of Korea; Biotechnology Process Engineering Center, KRIBB, Cheongju, 363-883, Republic of Korea.
Abstract:
Streptococcus parauberis is the major infectious agent of streptococcosis in the olive flounder (Paralichthys olivaceus), causing serious economic damage. In this study, we identified potential vaccine candidates against S. parauberis by reverse vaccinology. In total, the 2 out of 21 proteins were identified as vaccine candidates from two available S. parauberis genomes. The membrane-anchored protein SEC10/PgrA and the metal ABC transporter substrate-binding lipoprotein mtsA were potent antigenic proteins based on western blotting with mouse-derived antiserum against whole bacteria of S. parauberis serotypes I and II. In particular, metal ABC transporter substrate-binding lipoprotein (mtsA) showed similar protective immunity to that of whole-cell bacterins against S. parauberis in a zebrafish model. These results suggest that mtsA may be considered as a novel candidate in the development of vaccines against S. parauberis.
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