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Updated: Feb 1, 2026

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression
Published on: February 3, 2023
Synthesis of novel gallium-68 labeled rhodamine: A potential PET myocardial perfusion agent
I Al Jammaz1, B Al-Otaibi1, N Al-Hokbani1
1Cyclotron and Radiopharmaceuticals Department, King Faisal Specialist Hospital and Research Centre, P.O. Box 3354, Riyadh 11211, Saudi Arabia.
Abstract:
Myocardial perfusion imaging (MPI) is one of the most commonly performed investigations in nuclear medicine studies. Due to the physical properties of the positron emitter gallium-68 (68Ga) and its availability from 68Ge/68Ga generator, together with the well-known coordination chemistry, we have synthesized 68Ga-NOTA- and 68Ga-NODAGA-rhodamine conjugates using a straightforward and a one-step simple reaction. Radiochemical yields were greater than 95% (decay corrected), with total synthesis time of less than 30 min. Radiochemical purities were always greater than 98% as assessed by TLC and HPLC. These synthetic approaches hold considerable promise as simple method for 68Ga-rhodamine conjugates preparation, with high radiochemical yield and purity. Biodistribution studies in normal Fischer rats at 60 min post-injection, demonstrated significant (~3% ID/g) uptake and favorable biodistribution profile for 68Ga-NOTA-rhodamine over 68Ga-NODAGA-rhodamine conjugate. These results demonstrate that 68Ga-NOTA-rhodamine conjugate may be useful as probe for the PET evaluation of myocardial perfusion.
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