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Green Algae01:21

Green Algae

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Green algae, also referred to as chlorophytes, are different from red algae in having the chloroplasts containing chlorophylls a and b, which give them their distinct green hue. However, they lack phycobiliproteins, preventing them from developing the red or blue-green pigmentation seen in red algae. In terms of photosynthetic pigment composition, green algae closely resemble plants and share a close evolutionary relationship with them. Taxonomically Green algae belong to Phylum Chlorophyta in...
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Other Algae01:19

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The group Stramenopiles include some phototrophic microorganisms. Members of this group possess flagella covered in numerous short, hairlike extensions, a feature that inspired the group's name, derived from the Latin words for "straw" and "hair." Some of the main categories of Stramenopiles include diatoms, golden algae, and brown algae.Diatoms are unicellular, photosynthetic eukaryotes, with over 200 known genera. They play a key role in the planktonic communities of both marine and...
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Overview of Algae01:28

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The kingdom Archaeplastida encompasses red and green algae, along with land plants. Unlike other protists with chloroplasts that arose through secondary endosymbiosis, only red and green algae originated from primary endosymbiotic events. This diverse group of eukaryotic organisms contains chlorophyll and performs oxygenic photosynthesis.Algae exist in various forms, from large brown kelp in coastal waters to green scum in puddles and stains on rocks or soil. Some species are responsible for...
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Red Algae01:23

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Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
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What is Photosynthesis?00:39

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Photosynthesis is a multipart, biochemical process that occurs in plants as well as in some bacteria. It captures carbon dioxide and solar energy to produce glucose. Glucose stores chemical energy in the form of carbohydrates. The overall biochemical formula of photosynthesis is 6 CO2 + 6 H2O + Light energy → C6H12O6 + 6 O2. Photosynthesis releases oxygen into the atmosphere and is largely responsible for maintaining the Earth’s atmospheric oxygen content.
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Unlike carbon, water, and nitrogen, phosphorus is not present in the atmosphere as a gas. Instead, most phosphorus in the ecosystem exists as compounds, such as phosphate ions (PO43-), found in soil, water, sediment and rocks. Phosphorus is often a limiting nutrient (i.e., in short supply). Consequently, phosphorus is added to most agricultural fertilizers, which can cause environmental problems related to runoff in aquatic ecosystems.
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[FeFe]-hydrogenases from green algae.

Vera Engelbrecht1, Thomas Happe1

  • 1AG Photobiotechnologie, Fakultät für Biologie und Biotechnologie, Ruhr-Universität Bochum, Universitätsstraße, Bochum, Germany.

Methods in Enzymology
|December 5, 2018
PubMed
Summary

Microalgal hydrogenases offer sustainable hydrogen (H2) production. This chapter guides researchers on discovering, producing, and analyzing these enzymes for improved H2 generation.

Keywords:
ExpressionGreen algaeHydrogen production[FeFe]-hydrogenase

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Area of Science:

  • Biotechnology
  • Renewable Energy
  • Enzymology

Background:

  • Algal hydrogenases are efficient biocatalysts for hydrogen (H2) production using light energy.
  • Photobiological H2 evolution by microalgae is a sustainable alternative to fossil fuel-based H2 generation.
  • Discovering novel algal hydrogenases with unique properties can overcome limitations in H2 production.

Purpose of the Study:

  • To provide guidance on the discovery of novel algal hydrogenases.
  • To offer strategies for the heterologous production of active algal hydrogenase enzymes.
  • To detail methods for the biochemical and biophysical analysis of microalgal hydrogenases.

Main Methods:

  • Literature review on algal hydrogenase research.
  • Discussion of recombinant protein production challenges and solutions.
  • Overview of advanced techniques for enzyme characterization.

Main Results:

  • Algal hydrogenases possess unique features beneficial for H2 production.
  • Recombinant production of active hydrogenases remains a significant challenge.
  • Advanced analytical techniques require sufficient quantities of active enzyme.

Conclusions:

  • Overcoming the obstacle of obtaining sufficient active algal hydrogenase is crucial.
  • This chapter serves as a practical guide for researchers in the field.
  • Successful production and analysis of algal hydrogenases can advance sustainable H2 technology.