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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
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Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
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Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Related Experiment Video

Updated: Feb 1, 2026

Pupillary Response as Assessment of Effective Seizure Induction by Electroconvulsive Therapy
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Pupillary measurement during an assembly task.

Per Bækgaard1, Shahram Jalaliniya2, John Paulin Hansen3

  • 1DTU Compute, Technical University of Denmark, Richard Petersens Plads, Building 321, DK-2800 Kgs, Lyngby, Denmark.

Applied Ergonomics
|December 5, 2018
PubMed
Summary

Children

Keywords:
Assembly manualEye trackingPupil dilation

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Area of Science:

  • Human-Computer Interaction
  • Cognitive Science
  • Usability Engineering

Background:

  • Digital interfaces are increasingly common for instructional purposes.
  • Understanding user cognitive load is crucial for effective design.
  • Gaze tracking offers a non-invasive method to assess cognitive effort.

Purpose of the Study:

  • To compare the cognitive effort required for LEGO construction using printed booklets versus digital tablets.
  • To investigate the relationship between pupil dilation and task difficulty.
  • To explore the feasibility of using pupil size data in real-world scenarios.

Main Methods:

  • An empirical study involving 57 children assembling LEGO constructions.
  • Utilized head-mounted gaze trackers to monitor pupil dilation.
  • Compared performance and subjective feedback between printed booklet and digital tablet instruction groups.

Main Results:

  • Printed booklets elicited greater pupil dilation compared to digital tablets, especially as the initial medium.
  • Subjective reports indicated higher difficulty using the printed booklets.
  • Distinct pupil dilation patterns were observed in less productive children requiring more assistance.

Conclusions:

  • Pupil dilation can serve as a reliable indicator of cognitive effort and task difficulty in unconstrained settings.
  • Digital tablets may offer a more user-friendly instructional experience for LEGO construction compared to printed booklets.
  • Gaze tracking data holds potential for real-time assessment of user experience and cognitive load.