Honey bees are larger and live longer after developing at low temperature

Honey bees (Apis mellifera) are known to be temperature specialist and actively maintain brood temperature in a very narrow temperature range. Developing larvae are sensitive to changes of temperature in the nest. Temperatures lower than generally assumed as optimal have been shown to cause a number of negative developmental and behavioural changes in honey bees. We have reared both worker and drone larvae during the capped brood stage in cold (32 °C) and in warm temperatures (35 °C). Next, we measured their body mass at emergence and the longevity of individuals either caged in incubator (workers) or placed in maintaining colonies (drones). For drones, the reproductive caste, we also compared the mass and ratio of body parts (head, thorax, and abdomen) to body mass. As expected, both castes were heavier when reared in cold, but contrary to our expectations, both castes survived longer after emergence than bees reared in warm. Drones reared in cold were characterized by proportionally larger abdomens, in comparison to drones reared in warm.