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An Open-Source Plate Reader.

Karol P Szymula1, Michael S Magaraci1, Michael Patterson1

  • 1Department of Bioengineering , University of Pennsylvania , 210 South 33rd Street , Philadelphia , Pennsylvania 19104 , United States.

Biochemistry
|December 5, 2018
PubMed
Summary
This summary is machine-generated.

We developed an affordable, open-source microplate reader for biological research. This accessible instrument offers full-spectrum detection and optogenetic stimulation, empowering diverse experimental applications.

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Area of Science:

  • Experimental biology
  • Bioengineering
  • Synthetic biology
  • Optogenetics
  • Photosensory biology

Background:

  • Microplate readers are essential tools in biological research for multiplexed measurements.
  • Commercial plate readers are often prohibitively expensive, limiting accessibility for many researchers.
  • There is a growing need for cost-effective, open-source laboratory equipment.

Purpose of the Study:

  • To design, construct, and validate an open-source microplate reader.
  • To enhance accessibility of microplate reading technology.
  • To provide a low-cost alternative to commercial instruments.

Main Methods:

  • The system integrates full-spectrum absorbance and fluorescence detection.
  • It includes in situ optogenetic stimulation capabilities.
  • Stand-alone touch screen programming enables automated assay protocols.

Main Results:

  • The open-source microplate reader costs less than $3500.
  • It can detect fluorescence of common dyes at concentrations as low as ~10 nM.
  • Demonstrated applications include bacterial quorum sensing and LOV domain photoreceptor kinetics.

Conclusions:

  • This open-source microplate reader significantly reduces costs while maintaining functional capabilities.
  • The device supports diverse applications in synthetic biology, optogenetics, and photosensory biology.
  • Detailed assembly and automation guides facilitate widespread adoption and contribute to open-source biology hardware infrastructure.