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Updated: Feb 1, 2026

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Published on: October 15, 2019
Molecular Threading-Dependent Mass Transport in Paper Origami for Single-Step Electrochemical DNA Sensors
Dekai Ye1,2, Li Li3, Zhenhua Li2
1Institute of Molecular Medicine, Renji Hospital, School of Medicine , Shanghai Jiao Tong University , Shanghai 200127 , China.
Abstract:
Molecular transport controls the efficiency of complex biological network systems such as cellular signaling system and cascade biomedical reaction. However, device fabrication for molecular sensing is often restricted by a low transport efficiency and complicated processing. Here, we report a molecular threading-dependent transport system using three-dimensional (3D) paper origami enabling the directional transport of biomolecules. We demonstrate that framework nucleic acid-based interface engineering allows orthogonal molecular recognition and enzymatic reaction with programmed order on site. We thus develop a single-step electrochemical DNA sensor for quantitative analysis with 1 picomolar sensitivity within 60 min. Our sensor can discriminate a mismatched target at the level of a single base mismatch. Our study shows a great potential toward the development of a biomimetic molecular transport system for point-of-care and precision diagnosis.
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