Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

4.7K
Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell...
4.7K
Restorative Care01:19

Restorative Care

2.4K
Restorative care is provided once a patient has been discharged from a healthcare facility and requires additional services. The additional services include home care, rehabilitation programs, and extended care. Restorative care centers help the patient regain their previous level of functioning or acquire a new level of functioning due to the incapacitating effects of a disease or a disability. It aims to assist patients in enhancing their quality of life by encouraging independence,...
2.4K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

2.1K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
2.1K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Plant Cells and Tissues02:01

Plant Cells and Tissues

65.7K
Plant tissues are collections of similar cells performing related functions. Different plant tissues will have their own specialized roles and can be combined with other tissues to form organs such as flowers, fruit, stem, and leaves. Two major types of plant tissue include meristematic and permanent tissue.
65.7K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.5K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Human adipose ECM alleviates radiation-induced skin fibrosis via endothelial cell-mediated M2 macrophage polarization" [iScience, Volume 26, Issue 9 (2023) 107660].

iScience·2026
Same author

Proteomic atlas of human peritoneal tissue.

Scientific reports·2026
Same author

Adipose-derived stromal vascular fraction injection for radiation-induced bladder fibrosis: a first-in-human pilot study.

Stem cells translational medicine·2026
Same author

Adipose-Based Therapeutics and Transplantation of Hypodermis.

Advances in wound care·2025
Same author

Intravascular Lipoma.

Seminars in musculoskeletal radiology·2025
Same author

Nutritional Challenges in Post-Massive Weight Loss Body Contouring: Guidance for Plastic Surgeons on GLP-1 Agonists and Sleeve Gastrectomy.

Plastic and reconstructive surgery·2025

Related Experiment Video

Updated: Feb 1, 2026

Isolating Stem Cells from Soft Musculoskeletal Tissues
07:49

Isolating Stem Cells from Soft Musculoskeletal Tissues

Published on: July 5, 2010

13.9K

Amputation-Site Soft-Tissue Restoration Using Adipose Stem Cell Therapy.

Debra A Bourne1, R Dallin Thomas1, Jacqueline Bliley1

  • 1From the Departments of Plastic Surgery, Psychiatry, Radiology, Rehabilitation Science and Technology, and Bioengineering, University of Pittsburgh; the VA Pittsburgh Healthcare System; the University of Pittsburgh McGowan Institute of Regenerative Medicine; the Department of Physical Medicine and Rehabilitation, Walter Reed National Military Medical Center; and New York University Langone Medical Center.

Plastic and Reconstructive Surgery
|December 5, 2018
PubMed
Summary

Stem cell-enriched fat grafting is a safe new treatment for soft-tissue defects in lower extremity amputations. This pilot study showed over 60% volume retention, with cell viability linked to success.

More Related Videos

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy
06:00

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

Published on: February 3, 2023

4.2K
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

30.1K

Related Experiment Videos

Last Updated: Feb 1, 2026

Isolating Stem Cells from Soft Musculoskeletal Tissues
07:49

Isolating Stem Cells from Soft Musculoskeletal Tissues

Published on: July 5, 2010

13.9K
Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy
06:00

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

Published on: February 3, 2023

4.2K
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
09:20

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Published on: March 31, 2016

30.1K

Area of Science:

  • Regenerative Medicine
  • Orthopedic Surgery
  • Biomaterials Science

Background:

  • Soft-tissue deficits in amputation stumps cause pain and disability.
  • Stem cell-enriched fat grafting is an emerging treatment for soft-tissue reconstruction.
  • This is the first study to evaluate this technique in lower extremity amputations.

Purpose of the Study:

  • To assess the safety and efficacy of stem cell-enriched fat grafting for soft-tissue defects in lower extremity amputation stumps.
  • To evaluate graft volume retention, pain reduction, and functional improvement.

Main Methods:

  • Prospective cohort study of five military personnel with lower extremity amputations.
  • Fat grafting enriched with stromal vascular fraction (SVF) was performed.
  • Outcomes included complications, graft volume retention, cell viability, pain, and functional scores over 2 years.

Main Results:

  • No significant complications were observed.
  • Mean graft volume retention was 61.5 ± 24.0%.
  • Overall SVF cell viability correlated significantly with volume retention (p = 0.016).

Conclusions:

  • SVF-enriched fat grafting is a safe and novel treatment for symptomatic soft-tissue defects in traumatic lower extremity amputations.
  • Anticipated volume retention is approximately 60%.
  • Further research is necessary to confirm efficacy and optimize treatment protocols.