Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

57.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.9K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

1.9K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
1.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.1K
RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
Ladder Diagrams: Complexation Equilibria01:07

Ladder Diagrams: Complexation Equilibria

636
Ladder diagrams are useful for evaluating equilibria involving metal-ligand complexes. The vertical scale of the ladder diagram represents the concentration of unreacted or free ligand, pL. The horizontal lines on the scale depict the log of stepwise formation constants for metal-ligand complexes and indicate the dominant species in all the regions.
The formation constant, K1, for the formation of Cd(NH3)2+ complex from cadmium and ammonia is 3.55 × 102. Log K1 (i.e. pNH3) is 2.55, and...
636
Limitations of Friedel–Crafts Reactions01:26

Limitations of Friedel–Crafts Reactions

6.9K
Several restrictions limit the use of Friedel–Crafts reactions. First, the halogen in the alkyl halide must be attached to an sp3-hybridized carbon for the Friedel–Crafts reactions to occur. Vinyl or aryl halides do not react since the carbocations formed are unstable under the reaction conditions. Second, Friedel–Crafts alkylation is susceptible to carbocation rearrangement, and the major products obtained have a rearranged carbon skeleton. In contrast, the acylium ion is...
6.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mandibular Distraction Osteogenesis as Paradigm Shift for Treatment of Tongue-Based Obstructive Apnea: Results of a 16-Year, Hospital-Wide Intervention Trial.

Plastic and reconstructive surgery·2026
Same author

Patient-Reported Speech Outcomes in Patients with Beckwith-Wiedemann Syndrome.

Plastic and reconstructive surgery·2026
Same author

A small-molecule screen identifies that elafibranor links mechanical cues and Irf6-dependent epithelial differentiation.

Disease models & mechanisms·2026
Same author

The APERT Severity Scale: A Quantitative Tool for Risk Stratification in Apert Syndrome.

Plastic and reconstructive surgery·2025
Same author

Patient-Reported Psychosocial Outcomes in Patients with Beckwith-Wiedemann Syndrome.

Plastic and reconstructive surgery·2025
Same author

Applying Latent Profile Analysis to CLEFT-Q Data: A Framework for Understanding Patient Reported Outcomes in Cleft Care.

The Cleft palate-craniofacial journal : official publication of the American Cleft Palate-Craniofacial Association·2025

Related Experiment Video

Updated: Feb 1, 2026

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.5K

CRISPR Craft: DNA Editing the Reconstructive Ladder.

Danny S Roh1, Edward B-H Li1, Eric C Liao1

  • 1From Harvard Medical School; the Center for Regenerative Medicine; the Harvard Stem Cell Institute; and the Division of Plastic and Reconstructive Surgery, Massachusetts General Hospital.

Plastic and Reconstructive Surgery
|December 5, 2018
PubMed
Summary
This summary is machine-generated.

Clustered regularly interspaced short palindromic repeats (CRISPR) gene editing offers transformative potential across medical specialties, including plastic surgery. This review explores CRISPR

More Related Videos

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

4.0K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.9K

Related Experiment Videos

Last Updated: Feb 1, 2026

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.5K
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

4.0K
CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis
10:40

CRISPR Gene Editing Tool for MicroRNA Cluster Network Analysis

Published on: April 25, 2022

2.9K

Area of Science:

  • Genetics and Genomics
  • Molecular Biology
  • Biotechnology

Background:

  • The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system is a revolutionary genome editing technology.
  • CRISPR has broad implications across all fields of genetics and their applications.

Purpose of the Study:

  • To review the history and mechanism of CRISPR genome editing.
  • To highlight current and future applications of CRISPR technology.
  • To discuss the potential impact and use of CRISPR in plastic and reconstructive surgery.

Main Methods:

  • Literature review of CRISPR technology.
  • Analysis of CRISPR's applications in various medical fields.
  • Exploration of CRISPR's relevance to plastic surgery specialties.

Main Results:

  • CRISPR technology has significant potential for treating human diseases across clinical specialties.
  • Areas relevant to plastic surgery, including oncology, wound healing, immunology, and craniofacial malformations, can benefit from CRISPR.
  • The review covers CRISPR's mechanism, applications, and limitations.

Conclusions:

  • Plastic surgeons should become familiar with CRISPR gene editing technology.
  • Active contribution and leadership from plastic surgeons are encouraged for CRISPR applications.
  • CRISPR holds promise for advancing plastic and reconstructive surgery.