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Related Experiment Video

Updated: Feb 1, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

Process intensification for O₂ -dependent enzymatic transformations in continuous single-phase pressurized flow.

Juan M Bolivar^1,2, Alexander Mannsberger², Malene S Thomsen³

¹Austrian Centre of Industrial Biotechnology (ACIB), Graz, Austria.

Biotechnology and Bioengineering

|December 5, 2018

Summary

This summary is machine-generated.

Continuous-flow microreactors overcome oxygen supply limitations in oxidative biotransformations. This technology enhances enzyme kinetics and enables efficient, stable, and scalable chemical synthesis using oxygen.

Keywords:

flow microreactor homogeneous liquid phase oxidation oxygen-dependent transformation pressurized reactor reaction intensification

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Last Updated: Feb 1, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

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Published on: April 6, 2016

Measurements of Local Instantaneous Convective Heat Transfer in a Pipe - Single and Two-phase Flow

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Published on: April 30, 2018

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example

Published on: November 18, 2015

Area of Science:

Biocatalysis and Chemical Engineering
Process Intensification

Background:

Oxidative biotransformations are valuable for chemical synthesis but hindered by oxygen supply limitations.
Thermodynamic and kinetic constraints on oxygen transfer impede efficiency in fine chemical manufacturing.

Purpose of the Study:

To demonstrate continuous-flow microreactor technology as a solution for oxygen-dependent biotransformations.
To overcome process bottlenecks by operating at elevated pressures and dissolved oxygen concentrations.

Main Methods:

Utilized continuous-flow microreactors operating at medium pressures (up to 34 bar) to increase dissolved oxygen levels (up to 43 mM).
Employed glucose oxidase and d-amino acid oxidase reactions as model systems.
Incorporated catalase for oxygen recycling from hydrogen peroxide and co-immobilized enzymes in a packed-bed reactor.

Main Results:

Achieved up to six-fold reaction rate enhancement due to increased oxygen concentrations.
Doubled product formation by recycling oxygen with catalase, independent of gas-phase transfer.
Obtained high product concentrations (up to 80 mM) with stable catalyst performance over 360 cycles in a packed-bed reactor.

Conclusions:

Pressurized microreactors effectively overcome oxygen supply limitations in enzymatic conversions.
This technology enables process intensification for efficient and scalable biocatalytic synthesis.
Demonstrated a practical engineering solution for utilizing oxygen as a cosubstrate in enzymatic reactions.