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Related Experiment Video

Updated: Feb 1, 2026

Protein Complex Affinity Capture from Cryomilled Mammalian Cells
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Capturing Complex Enhancer Regulatory Hubs? Try Tri-C.

Wazeer Varsally1, Suzana Hadjur1

  • 1Research Department of Cancer Biology, UCL Cancer Institute, 72 Huntely Street, London WC1E 6BT, UK.

Developmental Cell
|December 5, 2018
PubMed
Summary

A new method called Tri-C enables sensitive, high-resolution study of multi-way chromatin interactions. This technique explores regulatory element interactions for precise gene regulation, particularly in the globin locus.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Genomics

Background:

  • Understanding gene regulation requires studying interactions between regulatory elements.
  • Allelic-level analysis provides crucial insights into precise gene control.

Purpose of the Study:

  • To introduce and validate Tri-C, a novel method for high-sensitivity, high-resolution allelic analysis of multi-way chromatin interactions.
  • To investigate combinatorial interactions among regulatory elements governing the globin locus.

Main Methods:

  • Development of Tri-C, a technique for mapping multi-way chromatin interactions.
  • Application of Tri-C to study the allelic landscape of the globin locus.

Main Results:

  • Tri-C achieves high sensitivity and resolution in detecting chromatin interactions.
  • The study elucidates combinatorial interactions of regulatory elements at the globin locus.

Conclusions:

  • Tri-C is a powerful new tool for dissecting complex gene regulatory networks.
  • The method advances the understanding of gene regulation at the allelic level.