Jove
Visualize
Contact Us

Related Concept Videos

The Blood-brain Barrier00:49

The Blood-brain Barrier

53.0K
Overview
53.0K
Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model01:09

Theories of Dissolution: The Danckwerts' Model and Interfacial Barrier Model

802
Various dissolution theories provide insight into the factors that influence the dissolution rate. Danckwerts' Model suggests that turbulence, rather than a stagnant layer, characterizes the dissolution medium at the solid-liquid interface. In this model, the agitated solvent contains macroscopic packets that move to the interface via eddy currents, facilitating the absorption and delivery of the drug to the bulk solution. The regular replenishment of solvent packets maintains the...
802
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.2K
Overview
80.2K
Fluid Connective Tissues: Blood and Lymph01:20

Fluid Connective Tissues: Blood and Lymph

18.4K
Blood and lymph are fluid connective tissues. They contain cells, also known as formed elements, circulating in a liquid extracellular matrix, the plasma. The formed elements are derived from hematopoietic stem cells in the bone marrow. Blood and lymph connect all vital parts and carry nutrients, oxygen, and other essential molecules like antibodies.
Blood
The blood flows through blood vessels— arteries, capillaries, and veins. Blood plasma is primarily made of proteins, solutes, and...
18.4K
Physiological Barriers01:25

Physiological Barriers

5.2K
Physiological barriers are semi-permeable cellular structures restricting drug diffusion into intracellular compartments and tissues. There are six types of physiological barriers: blood endothelial, cell membrane, blood-brain, blood-cerebrospinal fluid (CSF), blood-placenta, and blood-testis barriers.
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...
5.2K
Mucosal Barrier of the Stomach01:25

Mucosal Barrier of the Stomach

2.3K
The gastric glands contain parietal cells that secrete hydrochloric acid (HCl) for digestion. The cells secrete HCl because it is highly corrosive and essential for breaking down food. To achieve this, they secrete hydrogen and chloride ions into the lumen of the gastric glands, which combine to form HCl.
Within parietal cells, carbonic acid is first formed through the reaction of water and carbon dioxide. The dissociation of carbonic acid releases bicarbonate and hydrogen ions. The bicarbonate...
2.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scn4b Modulates Huntington's Disease Phenotype Severity in vivo.

bioRxiv : the preprint server for biology·2026
Same author

Strategies for blood-brain barrier rejuvenation and repair.

Nature reviews. Drug discovery·2026
Same author

A tissue-engineered endothelial cell - smooth muscle cell arteriole-like model.

Biomaterials science·2025
Same author

Aged human serum induces vascular changes in an isogenic co-culture venule model.

Stem cell reports·2025
Same author

Cytoplasmic anillin and Ect2 promote RhoA/myosin II-dependent confined migration and invasion.

Nature materials·2025
Same author

Extravasation of Borrelia burgdorferi Across the Blood-Brain Barrier is an Extremely Rare Event.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 1, 2026

Author Spotlight: Advancing the Use of Tissue Chip Technology for Studying Human Tissues
09:10

Author Spotlight: Advancing the Use of Tissue Chip Technology for Studying Human Tissues

Published on: January 12, 2024

3.7K

Benchmarking in vitro tissue-engineered blood-brain barrier models.

Jackson G DeStefano1,2, John J Jamieson1,3, Raleigh M Linville1,4

  • 1Institute for Nanobiotechnology, Johns Hopkins University, Baltimore, MD, USA.

Fluids and Barriers of the CNS
|December 6, 2018
PubMed
Summary

This study proposes key parameters for validating in vitro blood-brain barrier (BBB) models. These benchmarks, based on human and animal data, ensure in vitro models accurately reflect in vivo BBB characteristics for research.

Keywords:
BenchmarkingBlood–brain barrierBrain microvascular endothelial cellsIn vitro modelingInduced pluripotent stem cellsTissue-engineering

More Related Videos

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
09:23

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Published on: September 24, 2017

15.5K
Author Spotlight: A Personalized Approach Towards Investigating Alzheimer's Disease Using an In Vitro Blood-Brain Barrier Model
06:19

Author Spotlight: A Personalized Approach Towards Investigating Alzheimer's Disease Using an In Vitro Blood-Brain Barrier Model

Published on: October 20, 2023

2.9K

Related Experiment Videos

Last Updated: Feb 1, 2026

Author Spotlight: Advancing the Use of Tissue Chip Technology for Studying Human Tissues
09:10

Author Spotlight: Advancing the Use of Tissue Chip Technology for Studying Human Tissues

Published on: January 12, 2024

3.7K
Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
09:23

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Published on: September 24, 2017

15.5K
Author Spotlight: A Personalized Approach Towards Investigating Alzheimer's Disease Using an In Vitro Blood-Brain Barrier Model
06:19

Author Spotlight: A Personalized Approach Towards Investigating Alzheimer's Disease Using an In Vitro Blood-Brain Barrier Model

Published on: October 20, 2023

2.9K

Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Cell Biology

Background:

  • The blood-brain barrier (BBB) is crucial for brain homeostasis and is implicated in various diseases.
  • In vitro models of the BBB are vital research tools, but their accuracy is difficult to assess.
  • Existing knowledge of BBB structure and function largely stems from in vitro studies, complicating model validation.

Purpose of the Study:

  • To establish a foundational set of parameters for benchmarking and validating in vitro blood-brain barrier models.
  • To ensure in vitro models accurately recapitulate the structure and function of the in vivo BBB.

Main Methods:

  • Defined parameters related to BBB structure (ultrastructure, wall shear stress, geometry).
  • Included parameters for microenvironment (basement membrane, extracellular matrix).
  • Incorporated parameters for barrier function (TEER, permeability, efflux transport), cell function (marker expression, turnover), and co-culture components (astrocytes, pericytes).

Main Results:

  • Proposed a comprehensive set of parameters for BBB model validation.
  • Emphasized reliance on imaging and direct measurements from humans and animal models for benchmark setting.
  • Provided a framework for assessing the fidelity of in vitro BBB models.

Conclusions:

  • Standardized benchmarking parameters are essential for advancing the utility of in vitro BBB models.
  • Validation against in vivo data is critical for reliable BBB research.
  • These parameters offer a starting point for consistent and accurate BBB model development and evaluation.