Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mutations01:39

Mutations

94.5K
Overview
94.5K
The Tree of Life - Bacteria, Archaea, Eukaryotes02:40

The Tree of Life - Bacteria, Archaea, Eukaryotes

38.4K
The “tree of life” describes the evolution of life and the evolutionary relationships between organisms. The root of the tree is the common ancestor to all life on Earth. All other species radiate from this point, much like the branches of a tree. The numerous tips of these branches on the tree of life represent every living, or extant, species. Extinct species, which are species that no longer exist, can be found towards the center of the tree. Currently, these organisms, both...
38.4K
Exceptions to the Octet Rule02:55

Exceptions to the Octet Rule

37.6K
Many covalent molecules have central atoms that do not have eight electrons in their Lewis structures. These molecules fall into three categories:
37.6K
Viral Mutations00:36

Viral Mutations

39.9K
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...
39.9K
Lewis Symbols and the Octet Rule02:36

Lewis Symbols and the Octet Rule

80.8K
Chemical bonds are complex interactions between two or more atoms or ions, which reduce the potential energy of the molecule. Gilbert N. Lewis developed a model called the Lewis model that simplified the depiction of chemical bond formation and provided straightforward explanations for the chemical bonds seen in most common compounds.
80.8K
The Aufbau Principle and Hund's Rule03:02

The Aufbau Principle and Hund's Rule

72.8K
To determine the electron configuration for any particular atom, we can build the structures in the order of atomic numbers. Beginning with hydrogen, and continuing across the periods of the periodic table, we add one proton at a time to the nucleus and one electron to the proper subshell until we have described the electron configurations of all the elements. This procedure is called the aufbau principle, from the German word aufbau (“to build up”). Each added electron occupies the...
72.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synergistic integration of Fe-MOF and biomass-derived activated carbon for enhanced ciprofloxacin adsorption: experimental insights and DFT-based mechanistic elucidation.

RSC advances·2026
Same author

Preventive efficacy of oxygen therapy against contrast-associated acute kidney injury in patients undergoing coronary angiography: a systematic review and meta-analysis of randomized controlled trials.

BMC nephrology·2026
Same author

Prevalence and Risk Factors of Preserved Ratio Impaired Spirometry in People With HIV and Matched Population Control Participants.

Chest·2026
Same author

Risk-based breast cancer screening.

British journal of cancer·2026
Same author

Large-scale association study identifies lung cancer susceptibility copy number variants and their potential functional role in genetic instability.

medRxiv : the preprint server for health sciences·2026
Same author

Osteoarthritis and analgesic consumption in haemochromatosis HFE C282Y homozygotes with normal or low iron parameters.

Nature communications·2026

Related Experiment Video

Updated: Feb 1, 2026

A Simple Planting Technique for Re-establishing Trees Where Frequent Inundation Occurs
04:41

A Simple Planting Technique for Re-establishing Trees Where Frequent Inundation Occurs

Published on: January 26, 2018

6.6K

JAK2-tree: a simple CBC-based decision rule to guide appropriate JAK2 V617F mutation testing.

Etienne Mahe1, Kasper Mønsted Pedersen2, Yunus Çolak2

  • 1Division of Haematology, Alberta Public Laboratories, South Zone & Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada etienne.mahe@medportal.ca.

Journal of Clinical Pathology
|December 6, 2018
PubMed
Summary

A new JAK2-tree clinical decision rule aids in selecting patients for JAK2 V617F mutation testing. This approach optimizes laboratory resource utilization by identifying the most appropriate candidates for this myeloproliferative neoplasm test.

Keywords:
clinical auditlaboratory managementlaboratory testsmolecular pathologymyeloproliferative disease

More Related Videos

Simple and Computer-assisted Olfactory Testing for Mice
06:40

Simple and Computer-assisted Olfactory Testing for Mice

Published on: June 15, 2015

10.9K
A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis
08:34

A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis

Published on: June 12, 2014

10.3K

Related Experiment Videos

Last Updated: Feb 1, 2026

A Simple Planting Technique for Re-establishing Trees Where Frequent Inundation Occurs
04:41

A Simple Planting Technique for Re-establishing Trees Where Frequent Inundation Occurs

Published on: January 26, 2018

6.6K
Simple and Computer-assisted Olfactory Testing for Mice
06:40

Simple and Computer-assisted Olfactory Testing for Mice

Published on: June 15, 2015

10.9K
A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis
08:34

A Simple Behavioral Assay for Testing Visual Function in Xenopus laevis

Published on: June 12, 2014

10.3K

Area of Science:

  • Hematology
  • Molecular Diagnostics
  • Oncology

Background:

  • The JAK2 V617F mutation is a key marker in myeloproliferative neoplasms.
  • Current JAK2 V617F testing is sensitive but may be overutilized for screening.
  • Optimizing test selection is crucial for efficient laboratory resource management.

Purpose of the Study:

  • To develop a clinical decision rule for appropriate JAK2 V617F testing.
  • To improve patient selection for JAK2 V617F mutation analysis.
  • To reduce unnecessary laboratory testing for myeloproliferative neoplasms.

Main Methods:

  • A clinical decision rule, "JAK2-tree", was created.
  • The rule utilizes basic complete blood count (CBC) parameters: hemoglobin, platelet, and white blood cell counts.
  • JAK2-tree was validated on two independent datasets.

Main Results:

  • The JAK2-tree demonstrated high sensitivity for JAK2 V617F detection (91% and 94% on independent datasets).
  • Application of the JAK2-tree algorithm could reduce JAK2 V617F testing volume by 15% in a historical clinical laboratory setting.
  • The decision rule is easily applicable to routine CBC data.

Conclusions:

  • A simple, decision-tree-based screening approach effectively optimizes patient selection for JAK2 V617F testing.
  • The JAK2-tree facilitates appropriate utilization of diagnostic resources for myeloproliferative neoplasms.
  • This method supports evidence-based ordering of JAK2 V617F mutation tests.