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Updated: Feb 1, 2026

Enrichment for Chemoresistant Ovarian Cancer Stem Cells from Human Cell Lines
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Myotilin, a New Topotecan Resistant Protein in Ovarian Cancer Cell Lines

Karolina Sterzyńska1, Andrzej Klejewski2,3, Karolina Wojtowicz1

  • 1Department of Histology and Embryology, Poznan University of Medical Sciences, Poznań, Poland.

Journal of Cancer
|December 7, 2018
PubMed

Insights

Drug resistance limits chemotherapy effectiveness in ovarian cancer. This study found increased MYOT expression in topotecan-resistant ovarian cancer cells, suggesting a link to treatment resistance.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Chemotherapy in ovarian cancer faces challenges due to acquired drug resistance.
  • Topotecan (TOP) is a key drug in second-line ovarian cancer treatment, but resistance limits its efficacy.
  • Cancer cells develop diverse resistance mechanisms, impacting treatment outcomes.

Purpose of the Study:

  • To investigate the role of MYOT expression in the development of topotecan resistance in ovarian cancer.
  • To analyze MYOT mRNA and protein levels in topotecan-resistant ovarian cancer cell lines.

Main Methods:

  • Analysis of MYOT messenger RNA (mRNA) levels using real-time PCR.
  • Assessment of MYOT protein expression in cell lysates and culture media via western blot.
  • Immunofluorescence analysis to determine MYOT protein localization in cancer cells.

Main Results:

  • Elevated MYOT expression was observed at both mRNA and protein levels in topotecan-resistant ovarian cancer cell lines.
  • MYOT, along with extracellular matrix proteins COL1A2 and COL15A1, was detected in cell culture media.
  • Increased MYOT expression correlates with topotecan resistance in ovarian cancer models.

Conclusions:

  • Upregulation of MYOT is associated with topotecan resistance in ovarian cancer.
  • MYOT may play a role in the mechanisms underlying chemoresistance in ovarian cancer.
Keywords:
myotilinovarian cancertopotecan resistance

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