GFAP expression is influenced by astrocytoma grade and rs2070935 polymorphism
Mantas Sereika1, Ruta Urbanaviciute1, Arimantas Tamasauskas1
1Laboratory of Molecular Neurooncology, Neuroscience Institute, Medical Academy, Lithuanian University of Health Sciences, Eiveniu str. 4, Kaunas, LT 50009, Lithuania.
Journal of Cancer
|December 7, 2018
Summary
Glial fibrillary acidic protein (GFAP) expression and its rs2070935 polymorphism decrease with higher astrocytoma grade. The rs2070935 AA genotype is linked to poor prognosis in grade IV astrocytoma patients.
Area of Science:
- Neuroscience
- Molecular Biology
- Genetics
Background:
- Glial fibrillary acidic protein (GFAP) is crucial for astrocyte mechanical support.
- Astrocytomas are primary brain tumors originating from astrocytes.
- Genetic variations, like single nucleotide polymorphisms (SNPs), can influence gene expression and disease outcomes.
Purpose of the Study:
- To investigate GFAP expression at mRNA and protein levels in human astrocytoma samples.
- To analyze the association of the GFAP rs2070935 polymorphism with GFAP expression and astrocytoma grade.
- To explore the correlation between GFAP expression, rs2070935 genotypes, and patient survival.
Main Methods:
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for GFAP mRNA quantification.
- Western blot assay to measure GFAP protein levels.
- Quantitative PCR (qPCR) with TaqMan probes for rs2070935 genotyping.
Main Results:
- GFAP mRNA and protein expression significantly decreased with increasing astrocytoma grade (p < 0.05).
- A trend suggested increased GFAP mRNA expression correlated with shorter survival in grade IV astrocytoma patients (p = 0.2117).
- The rs2070935 CC genotype was linked to higher GFAP translational activity in grade II astrocytoma (p = 0.0238), while the AA genotype correlated with poor outcomes in grade IV patients (p = 0.0007).
Conclusions:
- GFAP expression declines with astrocytoma progression.
- The GFAP rs2070935 polymorphism may influence GFAP expression and clinical outcomes in astrocytoma.
- Further studies with larger sample sizes are needed to validate these findings, particularly the association between rs2070935 genotypes and patient prognosis.
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