Proteomic Profiling for Target Identification of Biologically Active Small Molecules Using 2D DIGE
Makoto Muroi1, Hiroyuki Osada2
1Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan.
Abstract:
Recent improvements in technologies such as omics analysis have enabled us to acquire a large amount of data regarding the biological changes in cells treated with bioactive small molecules. Using such data, a variety of profiling methods have been established for target identification of such bioactive compounds. In this chapter, we describe a proteomic profiling system, ChemProteoBase, based on proteome analysis using two-dimensional difference gel electrophoresis. This system compares the similarities in protein expression of 296 spots detected in the gel among the test compounds.
Related Concept Videos
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
Biological Effects of Radiation
What is Conservation Biology?
Molecules and Compounds
Activation Energy
Positive Regulator Molecules


