Quenching of Luminol Fluorescence at Nano-Bio Interface: Towards the Development of an Efficient Energy Transfer...

Surface modified colloidal gold (Au) and silver (Ag) nanoparticles (NPs) were used as efficient quenchers of luminol (LH₂) fluorescence either in homogeneous aqueous medium or its noncovalent assembly with bovine serum albumin (BSA). The mechanism as well as the extent of fluorescence quenching was found to be strongly dependent on the nature of the nanoparticles. While simple static type fluorescence quenching mechanism was perceived with AuNP, a more complex protocol involving quenching sphere model was envisaged for AgNP quenching. Nevertheless, the magnitude of Stern-Volmer (SV) quenching constant (K_SV ~ 10⁸-10¹⁰ M^-1) was calculated to be ca. 10⁴ times more for surface quoted NPs in comparison with BSA-NP bioconjugates system. On the other hand, a highly efficient (E ≈ 95%) energy transfer (ET) process was predicted for LH₂ captured in the hydrophobic assembly with BSA in presence of AgNP as an acceptor. The ET efficiency is critically dependent on the concentration of BSA and nicely correlated with the extent of NP surface coverage. However, fluorescence quenching on AuNP surface is relatively less responsive towards protein concentration, primarily due to the difference in surface activity as well as the mode of interaction of the protein with NPs. Graphical Abstract Energy transfer from excited luminol to metal nanoparticles is strongly modulated in presence of serum albumins.