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Role of FGF signalling in neural crest cell migration during early chick embryo development

Xiao-Tan Zhang1, Guang Wang1, Yan Li1

  • 11Department of Histology and Embryology, International Joint Laboratory for Embryonic Development & Prenatal Medicine, Medical College,Jinan University,Guangzhou 510632,China.

Zygote (Cambridge, England)
|December 7, 2018
PubMed

Insights

Blocking fibroblast growth factor (FGF) signaling enhances neural crest cell (NCC) migration during embryonic development. This regulation occurs via the cell cycle, specifically through cyclin D1, rather than altering N-cadherin expression.

Area of Science:

  • Developmental Biology
  • Cell Signaling

Background:

  • Fibroblast growth factor (FGF) signaling is a key regulator of neural crest cell (NCC) migration.
  • The precise mechanisms by which FGF signaling influences NCC migration remain incompletely understood.

Purpose of the Study:

  • To investigate the role of FGF signaling in modulating NCC migration.
  • To elucidate the molecular pathways involved in FGF-mediated regulation of NCC migration during neurulation.

Main Methods:

  • Transfection of neural tube cells with constructs to induce Sprouty2 (Spry2) or dominant-negative FGFR1 (Dn-FGFR1).
  • Analysis of NCC migration at cranial and trunk levels in developing embryos following blockage of FGF signaling at the HH10 stage.

Main Results:

  • Blocking FGF signaling at the neurulation stage significantly enhanced NCC migration.
  • FGF signaling did not affect N-cadherin expression in the neural tube.
  • Upregulation of Spry2 correlated with cyclin D1 overexpression in the dorsal neural tube, indicating a link to the cell cycle.

Conclusions:

  • FGF signaling regulates NCC migration during neurulation.
  • The cell cycle, specifically via cyclin D1, is a downstream target of FGF signaling in this process.
Keywords:
Cell cycleEMTFGF signallingNeural crest cellsSprouty2

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