Related Experiment Video
Updated: Feb 1, 2026
13:11
Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
Published on: May 20, 2010
13.1K
BIOINFORMATIC ANALYSIS OF DOSE- AND TIME-DEPENDENT miRNome RESPONSES
G Babini1, B Tanno2, I De Stefano3
1Physics Department, University of Pavia, Pavia, Italy.
Radiation Protection Dosimetry
|December 7, 2018
Summary
New
Area of Science:
- Radiobiology
- Molecular Biology
- Genomics
Background:
- 'Omics' technologies have advanced molecular analysis in cells.
- Low-dose radiation effects require further investigation.
- MicroRNAs (miRNAs) play crucial roles in cellular regulation.
Purpose of the Study:
- To investigate radiation-induced changes in miRNA profiles.
- To analyze the temporal dynamics of these miRNA alterations.
- To identify potential miRNA biomarkers for radiation exposure.
Main Methods:
- Human Umbilical Vein Endothelial Cells were exposed to low-dose gamma radiation.
- miRNA sequencing was performed at various time points post-irradiation.
- Bioinformatic and pathway enrichment analyses were conducted on differentially expressed miRNAs.
Main Results:
- Identified dose- and time-dependent alterations in miRNA expression profiles.
- Revealed specific pathways and functions affected by radiation exposure.
- Highlighted candidate miRNAs as potential biomarkers for low-dose radiation.
Conclusions:
- Low-dose gamma radiation significantly perturbs cellular miRNA profiles.
- miRNA expression dynamics provide insights into cellular responses to radiation.
- Candidate miRNAs show promise as biomarkers for assessing radiation exposure and its biological impact.
More Related Videos
14:56Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
5.2K
09:09Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics
Published on: October 13, 2020
5.1K
Related Concept Videos
01:03Dose-Response Relationship: Overview
5.1K
Agonists can bind with and activate receptors, resulting in the formation of drug-receptor complexes. Once formed, these complexes catalyze many biochemical processes at the cellular level and subsequently induce a pharmacologic response. The degree of response is directly proportional to the fraction of activated receptors, which in turn, depends on the concentration of the drug at the receptor site as well as the sensitivity of the receptor. An increase in the administered dose contributes to...
5.1K
02:39The Integrated Rate Law: The Dependence of Concentration on Time
41.6K
While the differential rate law relates the rate and concentrations of reactants, a second form of rate law called the integrated rate law relates concentrations of reactants and time. Integrated rate laws can be used to determine the amount of reactant or product present after a period of time or to estimate the time required for a reaction to proceed to a certain extent. For example, an integrated rate law helps determine the length of time a radioactive material must be stored for its...
41.6K
01:20Chronopharmacokinetics: Time-Dependent Pharmacokinetics
412
Chronopharmacokinetics studies the temporal change in drug absorption and elimination. These changes can be cyclical or non-cyclical. Cyclical changes occur over a regular interval, while non-cyclical changes occur over a longer, irregular period.
Time-dependent pharmacokinetics refers to non-cyclical changes in drug rate processes over a period of time. It can lead to nonlinear pharmacokinetics, where the relationship between drug concentration and time is not proportional. Non-cyclical...
Time-dependent pharmacokinetics refers to non-cyclical changes in drug rate processes over a period of time. It can lead to nonlinear pharmacokinetics, where the relationship between drug concentration and time is not proportional. Non-cyclical...
412
01:23Nonlinear Pharmacokinetics: Dependence of Elimination Half-Life and Dose Clearance
677
The elimination half-life and drug clearance of drugs following nonlinear kinetics can vary with dosage. The Michaelis-Menten parameters and drug concentration influence these factors. As the dose increases, the elimination half-life tends to lengthen, resulting in a reduction in clearance and a disproportionately larger area under the curve. The total clearance can be derived from the Michaelis-Menten equation for drugs following a one-compartment model.
A study on guinea pigs examined the...
A study on guinea pigs examined the...
677
01:22Dose-Response Relationship: Potency and Efficacy
6.5K
The potency of a drug is the measure of its ability to produce a biological response and can be compared by looking at the half-maximum effective concentration or EC50 values of different drugs. A lower EC50 value indicates higher potency of the drug. In the dose–response curve of two antihypertensive drugs, candesartan and irbesartan, a significant difference is observed in their EC50 values. A lower EC50 value for candesartan indicates that it is more potent than irbesartan, as it...
6.5K
01:25Dose-Response Relationship: Selectivity and Specificity
9.7K
Drugs exert their therapeutic effects by interacting with receptors, enzymes, or ion channels that are present throughout the human body. The strength and duration of the interaction between a drug and its target receptor are characterized by the selectivity and specificity of the drug. Selectivity refers to a drug's strong preference for its intended target over other targets. For instance, isoprenaline, a non-selective β-adrenergic agonist, interacts with both β1- and...
9.7K