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Yeast-like filamentous fungi: Molecular identification and in vitro susceptibility study

Maria Carmela Esposto1, Anna Prigitano1, Giuliana Lo Cascio2

  • 1Department of Biomedical Sciences for Health, Università degli Studi di Milano.

Medical Mycology
|December 7, 2018
PubMed

Insights

This study identified Magnusiomyces capitatus and M. clavatus in Italy, finding M. clavatus more common in blood infections. Their resistance to echinocandins may explain rising bloodstream infections.

Area of Science:

  • Medical Mycology
  • Clinical Microbiology

Background:

  • Yeast-like filamentous fungi are increasingly recognized as human pathogens.
  • Understanding the epidemiology and antifungal susceptibility of these fungi is crucial for effective treatment.

Purpose of the Study:

  • To identify yeast-like filamentous fungi from Italian clinical samples.
  • To determine the antifungal susceptibility profiles of identified species.
  • To investigate potential reasons for the increase in bloodstream infections.

Main Methods:

  • Molecular identification of fungal isolates.
  • Antifungal susceptibility testing using minimum inhibitory concentration (MIC) determination.
  • Retrospective analysis of isolate sources.

Main Results:

  • Magnusiomyces capitatus (28 isolates) and M. clavatus (18 isolates) were identified, with Geotrichum candidum (2 isolates) also found.
  • M. clavatus was prevalent in blood isolates (18/24), while M. capitatus predominated in other biological materials.
  • Intrinsic echinocandin resistance was confirmed for both M. capitatus and M. clavatus.
  • Both species showed low MICs for itraconazole, posaconazole, and voriconazole.
  • M. clavatus exhibited lower flucytosine MICs and higher isavuconazole MICs compared to M. capitatus.

Conclusions:

  • The intrinsic echinocandin resistance of M. capitatus and M. clavatus may contribute to the rise in M. clavatus bloodstream infections.
  • Targeted antifungal therapies with azoles or flucytosine may be considered for infections caused by these fungi.
Keywords:
GeotrichumMagnusiomycesSaprochaetefungemiayeast-like filamentous fungi

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