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Updated: Feb 1, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Tuning store-operated calcium entry to modulate Ca2+-dependent physiological processes
Hwei Ling Ong1, Krishna Prasad Subedi1, Ga-Yeon Son1
1Secretory Physiology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
This review details how stromal interaction molecules (STIM1/2) regulate calcium signaling via Orai1 and TRPC channels, crucial for cell functions. Understanding these calcium entry pathways is key to cellular regulation.
Area of Science:
- Cell Biology
- Molecular Physiology
- Biochemistry
Background:
- Intracellular calcium signaling is vital for diverse cell functions.
- Store-operated calcium entry (SOCE) relies on endoplasmic reticulum calcium levels.
- STIM proteins (STIM1/2) and Orai1 channels mediate SOCE.
Purpose of the Study:
- To review molecular components governing calcium (Ca2+) entry pathways.
- To discuss the regulation of SOCE and TRPC channels by STIM proteins.
- To highlight the spatiotemporal control of calcium signals.
Main Methods:
- This is a review article, synthesizing existing research.
- Focuses on molecular mechanisms of calcium channel regulation.
- Discusses protein interactions and cellular localization.
Main Results:
- STIM1 and STIM2 sense and respond to endoplasmic reticulum calcium depletion.
- STIM proteins recruit and activate Orai1 and some TRPC channels at ER-PM junctions.
- STIM proteins modulate SOCE sensitivity and channel function.
Conclusions:
- STIM proteins are central regulators of calcium influx.
- Orai1 and TRPC channels mediate distinct but overlapping calcium signaling roles.
- Precise regulation of calcium entry is essential for cellular homeostasis and function.
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