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Related Concept Videos

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Related Experiment Video

Updated: Feb 1, 2026

Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum
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Prominin-1 controls stem cell activation by orchestrating ciliary dynamics.

Donald Singer1, Kristina Thamm2, Heng Zhuang1,3

  • 1Peninsula Dental School, University of Plymouth, Plymouth, UK.

The EMBO Journal
|December 8, 2018
PubMed
Summary

Prominin-1 (Prom1) is essential for primary cilium function in stem cells, regulating their response to extracellular signals for proper development and regeneration. Its absence disrupts stem cell activation and maintenance.

Keywords:
CD133ciliasonic hedgehogstem cellstooth

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Stem cell activation relies on precise cell signaling.
  • The primary cilium acts as a cellular sensory organelle, transmitting external signals.
  • Ciliary dynamics are tightly regulated by the cell cycle.

Purpose of the Study:

  • To investigate the role of Prominin-1 (Prom1) in regulating ciliary dynamics within stem cells.
  • To understand how Prom1 influences stem cell response to extracellular signals like sonic hedgehog (SHH).

Main Methods:

  • Utilized mouse incisor tooth epithelia as a model system.
  • Examined the function of Prominin-1 (Prom1/CD133), a cholesterol-binding glycoprotein.
  • Observed nuclear translocation of Prom1 and associated molecules in transit amplifying cells.

Main Results:

  • Prom1 is crucial for maintaining primary cilium dynamics in stem cells.
  • Absence of Prom1 disrupts stem cell quiescence and activation.
  • Prom1 deficiency abolishes the growth-stimulating effects of SHH treatment.

Conclusions:

  • Prominin-1 is a key regulator of stem cell response to extracellular signals.
  • Proper ciliary dynamics controlled by Prom1 are vital for stem cell function.
  • Implications for understanding development, regeneration, and related diseases.