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Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Lipid-derived Compounds in the Human Body01:31

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Fats and lipids are crucial components in the human body. Some lipid-derived compounds, such as fat-soluble vitamins, eicosanoids, lipoproteins, and glycolipids, also play unique roles to support various  biological processes .
Fat-soluble Vitamins
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Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells
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Pericytes Derived from Human Pluripotent Stem Cells.

John Jamieson1, Bria Macklin1, Sharon Gerecht2

  • 1Department of Chemical and Biomolecular Engineering, Institute for NanoBioTechnology, and the Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD, USA.

Advances in Experimental Medicine and Biology
|December 8, 2018
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cells (hPSCs) offer a scalable method for generating pericytes in vitro. This research compares methods for hPSC-derived pericytes, crucial for vascular research and cell therapies.

Keywords:
BiomarkersDevelopmentDifferentiationEmbryoid bodyIn vitroMural cellsPericytesPluripotent stem cellsProtocolSpecificationTissue engineeringVasculature

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Area of Science:

  • Stem cell biology
  • Vascular biology
  • Regenerative medicine

Background:

  • Pericytes are crucial for microvessel development, stability, and function.
  • Pericytes are implicated in cancer growth and wound healing processes.
  • A consistent and scalable source of pericytes is needed for therapeutic and modeling applications.

Purpose of the Study:

  • To review and compare methods for differentiating pericytes from human pluripotent stem cells (hPSCs).
  • To discuss the characterization of hPSC-derived pericytes.
  • To explore the potential of hPSC-pericytes for downstream applications.

Main Methods:

  • Review of existing literature on pericyte differentiation from hPSCs.
  • Comparative analysis of different differentiation protocols.
  • Discussion of characterization techniques for hPSC-pericytes.

Main Results:

  • Human pluripotent stem cells (hPSCs) can be differentiated into pericytes efficiently in vitro.
  • Multiple approaches exist for generating hPSC-derived pericytes, with varying yields and specificities.
  • Characterization methods confirm the identity and purity of hPSC-pericytes.

Conclusions:

  • In vitro differentiation of pericytes from hPSCs provides a reliable and scalable cell source.
  • Studying hPSC-pericyte differentiation aids in understanding pericyte development and related cell types.
  • Defined hPSC-pericyte populations will advance tissue engineering and cell-based therapies.