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A novel autoantibody causing a peripheral fluorescent antinuclear antibody pattern is specific for nuclear pore
A Dagenais1, V Bibor-Hardy, J L Senécal
1Institut du Cancer de Montréal, Notre-Dame Hospital, Quebec, Canada.
Arthritis and Rheumatism
|October 1, 1988
Summary
Serum from a polymyositis patient showed a unique nuclear rim pattern on cells. This pattern localized to nuclear pore complexes, identifying 200- and 130-kd antigens in this autoimmune disease.
Area of Science:
- Cell Biology
- Immunology
- Autoimmunity
Background:
- Polymyositis is an autoimmune disease causing muscle inflammation.
- Antinuclear antibodies (ANAs) are common in autoimmune conditions.
- Characterizing novel ANA patterns aids in understanding disease mechanisms.
Observation:
- Serum from a polymyositis patient displayed a peripheral (rim) fluorescent antinuclear antibody pattern on rat liver cells.
- Indirect immunofluorescence showed a punctate pattern on the nuclear surface of PtK2, BHK-21, and HEp-2 cells.
- This pattern persisted after cellular extraction, indicating stable antigen association.
Findings:
- Immunoelectron microscopy localized the target antigens specifically to nuclear pore complexes.
- Immunoblot analysis identified two major antigenic polypeptides of 200 kDa and 130 kDa.
- These antigens were found to be enriched in the nuclear fraction of cells.
Implications:
- This study identifies novel nuclear pore complex antigens associated with polymyositis.
- Understanding these antigens may provide new diagnostic or therapeutic targets for autoimmune myopathies.
- The findings contribute to the broader knowledge of antinuclear antibodies and their cellular targets.