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Disintegration of microorganisms.

M D White1, D Marcus

  • 1Israel Institute for Biological Research, Ness Ziona.

Advances in Biotechnological Processes
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

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Choosing the right equipment for large-scale microbial cell disintegration is crucial. High-pressure homogenization and wet milling are common, but optimal conditions must be determined for each specific microorganism and product.

Area of Science:

  • Biotechnology
  • Microbiology
  • Bioprocessing Engineering

Background:

  • Large-scale microbial cell disintegration is essential for extracting intracellular products.
  • High-pressure homogenization and wet milling are the most prevalent industrial methods.
  • Limited published data exists for newer systems like microfluidization.

Purpose of the Study:

  • To review common methods for large-scale microbial cell disintegration.
  • To highlight factors influencing the choice of equipment and process conditions.
  • To emphasize the need for process optimization for each specific application.

Main Methods:

  • Review of established industrial cell disintegration techniques.
  • Discussion of equipment types including homogenizers (Manton Gaulin, Rannie) and ball mills (Dyno-Mill, Netzsch).

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  • Consideration of emerging technologies like microfluidization.

    • Main Results:

    • High-pressure homogenization and wet milling are widely adopted for large-scale cell disruption.
    • Several manufacturers offer equipment for these processes.
    • Factors for selecting optimal disintegration equipment and conditions are presented.

    Conclusions:

    • Mechanical disintegration methods are broadly applicable but require specific optimization.
    • Consulting existing data and conducting lab-scale experiments are vital for scale-up.
    • Tailoring disintegration conditions to each microorganism and product is essential for biotechnological applications.