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Updated: Feb 1, 2026
RNA Interference in Ticks
Published on: January 20, 2011
Differential expression of genes in greenbug (Schizaphis graminum Rondani) treated by imidacloprid and RNA
Bai-Zhong Zhang1,2, Kang-Sheng Ma2, Jun-Jie Liu1
1Department of Plant Protection, College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Poeple's Republic of China.
Background:
Insecticides act as toxins, inhibitors of digestion and deterrents, and affect the expression of many genes in insects. To assess key genes associated with the detoxification or regulation of imidacloprid in greenbug, Schizaphis graminum (Rondani), the transcriptome and digital gene expression (DGE) profile were analyzed using Illumina sequencing.
Results:
In total, 48 763 494 clean reads were obtained by sequencing. Expression profile analysis showed that 2782 unigenes were differently expressed between the imidacloprid treatment and control groups. After exposure to imidacloprid, the expression levels of 1846 unigenes were upregulated and 936 were downregulated in comparison with controls. Expression patterns of the top 20 highly expressed genes show that they could be involved in the detoxification of imidacloprid. Silencing of multidrug resistance-associated gene (MRA), GATA-binding gene (GAT) and takeout-like precursor gene (TLP) resulted in increasing susceptibility to imidacloprid.
Conclusions:
The differentially expressed genes in S. graminum have potential regulatory or detoxification roles in response to imidacloprid. These results should be useful in understanding the molecular mechanisms of greenbug adaption to imidacloprid. © 2018 Society of Chemical Industry.
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