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Related Experiment Videos

Long term liver preservation using artificial blood substitutes.

I Nakajima1, S Fuchinoue, S Teraoka

  • 1Department of Surgery and Therapeutic Engineering, Tokyo Women's Medical College, Japan.

Biomaterials, Artificial Cells, and Artificial Organs
|January 1, 1988
PubMed
Summary

Modified hemoglobin solutions (PHP-3 and PHP-4) significantly improved canine liver preservation for 24 hours, leading to complete functional recovery and extended survival after transplantation.

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Area of Science:

  • Organ transplantation
  • Biomedical engineering
  • Surgical research

Background:

  • Hypothermic perfusion preservation is critical for extending organ viability.
  • Artificial blood substitutes are being explored to enhance preservation solutions.
  • Current preservation methods require optimization for improved post-transplant outcomes.

Purpose of the Study:

  • To evaluate the efficacy of novel hemoglobin-based artificial blood substitutes for 24-hour canine liver preservation.
  • To compare the functional recovery and survival rates of transplanted livers preserved with different perfusates.

Main Methods:

  • Canine livers were preserved for 24 hours using four different perfusates: Oxypherol solution, Stabilized Hemoglobin (PHP-1), and two modified versions (PHP-3, PHP-4).
  • Orthotopic liver transplantation was performed after preservation.

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  • Postoperative graft function was assessed by monitoring bile excretion, consciousness level, activated clotting time, and survival rate.

    • Main Results:

    • Perfluorotributylamine (Oxypherol) and PHP-1 solutions showed limited improvement in graft function.
    • Modified hemoglobin solutions (PHP-3 and PHP-4) resulted in complete functional recovery of the transplanted livers.
    • The PHP-4 solution demonstrated the longest survival rate among the tested groups.

    Conclusions:

    • Modified hemoglobin-based solutions, particularly PHP-4, are effective perfusates for extended hypothermic liver preservation.
    • These novel solutions show promise for improving outcomes in liver transplantation.