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Protocol for Long Duration Whole Body Hyperthermia in Mice
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Protocol for Long Duration Whole Body Hyperthermia in Mice

Published on: August 25, 2012

Hyperthermia, antipyretics and function of polymorphonuclear leukocytes

T W Austin, G Truant

    Canadian Medical Association Journal
    |March 4, 1978
    PubMed

    Insights

    Hyperthermia (40°C) did not impair polymorphonuclear leukocyte (PMNL) function. However, common antipyretics like sodium salicylate and phenacetin were found to adversely affect PMNL migration and bacterial killing capabilities.

    Area of Science:

    • Immunology
    • Pharmacology

    Background:

    • Polymorphonuclear leukocytes (PMNLs) are crucial for innate immunity.
    • The impact of hyperthermia and common antipyretic drugs on PMNL function requires clarification.

    Purpose of the Study:

    • To investigate the effects of hyperthermia (40°C) and antipyretic drugs (salicylates, acetaminophen, phenacetin) on PMNL viability, migration, and bactericidal activity.

    Main Methods:

    • PMNL viability assessed using trypan blue exclusion.
    • Migration assays conducted in Boyden chambers with bacterial chemotactic factor.
    • Bactericidal assays performed using Staphylococcus aureus and human serum.

    Main Results:

    • Hyperthermia (40°C) and tested drugs did not adversely affect PMNL viability.
    • Sodium salicylate and phenacetin significantly suppressed PMNL migration.
    • Acetylsalicylic acid, sodium salicylate, and phenacetin impaired early and late staphylococcal killing.

    Conclusions:

    • PMNL function is generally maintained at 40°C compared to 37°C.
    • Commonly used antipyretic medications can adversely impact PMNL immune functions, including migration and bacterial clearance.

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