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Hyperthermia, antipyretics and function of polymorphonuclear leukocytes.
Canadian Medical Association Journal
|March 4, 1978
Summary
Hyperthermia (40°C) did not impair polymorphonuclear leukocyte (PMNL) function. However, common antipyretics like sodium salicylate and phenacetin were found to adversely affect PMNL migration and bacterial killing capabilities.
Area of Science:
- Immunology
- Pharmacology
Background:
- Polymorphonuclear leukocytes (PMNLs) are crucial for innate immunity.
- The impact of hyperthermia and common antipyretic drugs on PMNL function requires clarification.
Purpose of the Study:
- To investigate the effects of hyperthermia (40°C) and antipyretic drugs (salicylates, acetaminophen, phenacetin) on PMNL viability, migration, and bactericidal activity.
Main Methods:
- PMNL viability assessed using trypan blue exclusion.
- Migration assays conducted in Boyden chambers with bacterial chemotactic factor.
- Bactericidal assays performed using Staphylococcus aureus and human serum.
Main Results:
- Hyperthermia (40°C) and tested drugs did not adversely affect PMNL viability.
- Sodium salicylate and phenacetin significantly suppressed PMNL migration.
- Acetylsalicylic acid, sodium salicylate, and phenacetin impaired early and late staphylococcal killing.
Conclusions:
- PMNL function is generally maintained at 40°C compared to 37°C.
- Commonly used antipyretic medications can adversely impact PMNL immune functions, including migration and bacterial clearance.