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Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
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Analysis of differential gene expression by RNA-seq data in ABCG1 knockout mice
Si-Qi Shen1, Xiao-Wei Yan1, Peng-Tao Li2
1Department of Cardiology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing 100730, PR China.
Gene
|December 12, 2018
Summary
Accurate knockout of ATP-binding cassette transporter G1 (ABCG1) in mice altered numerous genes involved in lipid metabolism and atherosclerosis. This gene expression changes impact future research on ABCG1
Area of Science:
- Genetics and Molecular Biology
- Cardiovascular Research
- Immunology
Background:
- Previous studies on ATP-binding cassette transporter G1 (ABCG1) in atherosclerosis using genetically modified mice yielded inconsistent results.
- Accurate gene targeting is crucial for understanding the specific role of ABCG1 in disease pathogenesis.
Purpose of the Study:
- To investigate the transcriptional changes in atherosclerosis-related genes following precise knockout of ABCG1.
- To establish a reliable mouse model for studying ABCG1's function in atherosclerosis.
Main Methods:
- Generated a precise ABCG1 knockout mouse model on a C57BL/6 background.
- Performed whole transcriptome analysis (RNA-seq) on peripheral blood mononuclear cells from ABCG1 knockout and wildtype control mice.
Main Results:
- ABCG1 knockout resulted in differential expression of 605 genes, including 306 upregulated and 299 downregulated.
- Significant alterations were observed in genes related to lipid metabolism (13 genes), cell adhesion, leukocyte migration, and apoptosis.
- Enriched pathways included fatty acid biosynthesis, immune response, and intracellular signal transduction.
Conclusions:
- ABCG1 knockout mice display altered expression of multiple atherosclerosis-related genes.
- These transcriptional changes may influence the interpretation of future studies utilizing this model to elucidate ABCG1's role in atherosclerosis.
