Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

18.8K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.8K
System of Memory01:23

System of Memory

7.4K
Memory is categorized into three major systems: sensory memory, short-term memory (STM), and long-term memory (LTM). These systems differ in their capacity and the duration for which they can hold information. Sensory memory captures raw sensory input from the environment, holding it for just a few seconds or less. For example, on hearing a brief, loud sound, like a car horn honking, the sound seems to linger in the mind for a moment even after it stops. This is an instance of sensory memory...
7.4K
Working Memory01:24

Working Memory

875
Working memory refers to a combination of components, including short-term memory and attention, that allow an individual to hold information temporarily as we perform cognitive tasks. It is an essential cognitive function that enables the execution of complex tasks such as problem-solving, comprehension, and reasoning. Unlike short-term memory, which simply involves the storage of information for a brief period, working memory involves the active manipulation and processing of this...
875
Long-Term Memory01:18

Long-Term Memory

679
Long-term memory is a relatively permanent type of memory, capable of storing vast amounts of information over extended periods. Its storage capacity is generally considered unlimited.
Long-term memory can be categorized into two primary types: explicit and implicit memory. Explicit memory, also known as declarative memory, involves the conscious recollection of information that we deliberately try to remember, recall, and articulate. This type of memory encompasses specific facts, events, and...
679
Traumatic Memory01:20

Traumatic Memory

582
Emotionally traumatic events often lead to memories that are exceptionally vivid and enduring, sometimes persisting with remarkable clarity throughout an individual's life. A classic example of this phenomenon is a person who survives a car accident. Even years later, they may recall every detail of the event with startling accuracy — the screeching of the tires, the jarring impact, and the acrid smell of burning rubber. Such vividness contrasts sharply with how an individual...
582
Repressed Memory01:16

Repressed Memory

522
Repressed memories are a psychological phenomenon where memories of traumatic events are unconsciously blocked from a person's awareness. This process occurs as a defense mechanism, protecting the mind from the emotional impact of distressing or painful experiences. For example, a person who has experienced childhood trauma may grow up with no conscious recollection of the event. In such cases, the memories are thought to be buried deep within the subconscious, inaccessible to the conscious...
522

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Longitudinal mechanisms of response, resistance and relapse to teclistamab in multiple myeloma: results from MajesTEC-1.

Haematologica·2026
Same author

Human epidermal growth factor receptor 3-targeted near-infrared photoimmunotherapy in a xenograft mouse model of breast cancer.

BMC cancer·2026
Same author

Methylene blue protects hair follicle stem cells from oxidative and metabolic stress to enhance hair regeneration.

Aging·2026
Same author

Epidermal growth factor receptor-targeted near-infrared photoimmunotherapy for malignant pleural mesothelioma.

Lung cancer (Amsterdam, Netherlands)·2026
Same author

Understanding tumor microenvironment dynamics and immune checkpoint inhibitor efficacy following mEGFR-targeted near-infrared photoimmunotherapy.

Cancer letters·2026
Same author

Combined CD25-targeted near infrared photoimmunotherapy (NIR-PIT) and intratumor IL-15 enhance the effectiveness of anti-PD-1 immunotherapy.

Cancer immunology, immunotherapy : CII·2025

Related Experiment Video

Updated: Feb 1, 2026

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells
06:07

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Published on: March 28, 2025

1.2K

Eomesodermin driven IL-10 production in effector CD8+ T cells promotes a memory phenotype.

John Reiser1, Kavitha Sadashivaiah2, Aki Furusawa2

  • 1Program in Oncology, Greenebaum Cancer Center, Center for Stem Cell Research and Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD, United States; Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD, United States.

Cellular Immunology
|December 12, 2018
PubMed
Summary

Eomesodermin (Eomes) and Interleukin-10 (IL-10) promote central memory CD8+ T cell development in mice. Eomes boosts IL-10 and CD62L expression, enhancing T cell homing to lymph nodes for memory formation.

Keywords:
CD62LCentral-memory T cellEffector T cellEomesIL-10T-bet

More Related Videos

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
08:49

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Published on: March 18, 2020

11.6K
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
09:42

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

Published on: June 7, 2017

12.0K

Related Experiment Videos

Last Updated: Feb 1, 2026

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells
06:07

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Published on: March 28, 2025

1.2K
Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
08:49

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Published on: March 18, 2020

11.6K
A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue
09:42

A Chronic Autoimmune Dry Eye Rat Model with Increase in Effector Memory T Cells in Eyeball Tissue

Published on: June 7, 2017

12.0K

Area of Science:

  • Immunology
  • Cellular Biology
  • Molecular Biology

Background:

  • CD8+ T cell differentiation is regulated by transcription factors T-bet and Eomesodermin (Eomes).
  • Cytokines such as Interleukin-10 (IL-10) play critical roles in T cell function.
  • The interplay between T-box proteins and IL-10 in memory T cell fate determination is not fully understood.

Purpose of the Study:

  • To elucidate the mechanisms by which Eomes and IL-10 interact to promote a central memory CD8+ T cell phenotype.
  • To investigate the role of Eomes in IL-10 production and CD62L expression in effector CD8+ T cells.

Main Methods:

  • Murine CD8+ T cell differentiation models were utilized.
  • Analysis of transcription factor expression (Eomes) and cytokine signaling (IL-10).
  • Assessment of cell surface marker expression, including the lymph node homing selectin CD62L.

Main Results:

  • Eomes expression was found to drive a central memory phenotype in murine CD8+ T cells.
  • Eomes promoted increased IL-10 expression by effector CD8+ T cells.
  • Eomes led to elevated levels of CD62L, a lymph node homing marker.
  • IL-10 exposure maintained CD62L expression on effector CD8+ T cells in vitro.

Conclusions:

  • Eomes facilitates a stepwise transition of effector T cells towards a memory phenotype.
  • Eomes synergizes with IL-10 to enhance CD62L expression, promoting lymph node homing.
  • Early upregulation of homing markers by Eomes may aid effector T cell retention in secondary lymphoid organs, fostering central memory development.