Related Experiment Video
Updated: Feb 1, 2026
Investigating the Alleviating Effects of Bacillus cereus Administration on Colitis through Gut Microbiota Modulation
Published on: July 27, 2022
pVAX1-A20 alleviates colitis in mice by promoting regulatory T cells
Tianyong Hu1, Wenhui Hu2, Li Ma1
1Longgang ENT Hospital, Institute of ENT and Shenzhen Key Laboratory of ENT, Shenzhen, China.
Aim:
To investigate whether the intrarectal administration of the ubiquitin E3 ligase A20 (A20) attenuates intestinal inflammation and influences regulatory T cells in experimental colitis.
Methods:
A dextran sulfate sodium induced chronic colitis mouse model was established. The symptoms and manifestations of colitis and the severity of colonic mucosal inflammation were evaluated. The protective role of A20 expression in the intestine was analyzed after the administration of a pVAX1-A20 recombinant eukaryotic vector, which was encapsulated into poly(L-lactide-co-glycolide) as a nanoparticle.
Results:
pVAX1-A20 administration markedly ameliorated colonic tissue damage and reduced intestinal inflammation via the suppression of the mucosal mitogen-activated protein kinase and nuclear factor (NF)-κB signaling cascade. Furthermore, pVAX1-A20 promoted the splenic regulatory T cell population and forkhead box P3 expression in colonic tissue.
Conclusion:
A20 plays a key role in the regulation of intestinal inflammation and that the overexpression of A20 in the intestine protects mice from dextran sulfate sodium induced chronic colitis.
Insights
Intrarectal administration of ubiquitin E3 ligase A20 (A20) significantly reduced intestinal inflammation and tissue damage in experimental colitis. This treatment also enhanced regulatory T cells, offering a protective effect against dextran sulfate sodium-induced colitis.
Area of Science:
- Immunology
- Gastroenterology
- Molecular Biology
Background:
- Intestinal inflammation is a hallmark of inflammatory bowel diseases.
- Regulatory T cells (Tregs) play a crucial role in maintaining immune homeostasis in the gut.
- Ubiquitin E3 ligase A20 (A20) is implicated in modulating inflammatory responses.
Purpose of the Study:
- To determine if intrarectal administration of A20 can reduce intestinal inflammation in experimental colitis.
- To investigate the effect of A20 on regulatory T cells in the context of colitis.
Main Methods:
- A chronic colitis mouse model was induced using dextran sulfate sodium (DSS).
- A recombinant eukaryotic vector encoding A20 (pVAX1-A20) was formulated into nanoparticles for intrarectal delivery.
- Colitis severity, inflammation markers, and Treg populations were assessed.
Main Results:
- pVAX1-A20 administration significantly ameliorated colonic tissue damage and reduced intestinal inflammation.
- Treatment suppressed the mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-κB) signaling pathways.
- A20 overexpression promoted splenic regulatory T cell numbers and forkhead box P3 (FOXP3) expression in colonic tissue.
Conclusions:
- A20 plays a critical role in regulating intestinal inflammation.
- Intrarectal A20 delivery protects against DSS-induced chronic colitis by modulating inflammatory signaling and enhancing Treg populations.
More Related Videos
Related Concept Videos
02:02Cis-regulatory Sequences
02:02Cis-regulatory Sequences
02:40The Eukaryotic Promoter Region
02:40The Eukaryotic Promoter Region
01:28Global Regulatory Systems
00:50Anaphase Promoting Complex