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Updated: Feb 1, 2026

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
Published on: January 5, 2021
A sensitive and accurate vortex-assisted liquid-liquid microextraction-gas chromatography-mass spectrometry method
Simone Caetani Machado1, Bruna Maciel Souza1, Luiz Paulo de Aguiar Marciano1
1Laboratory of Toxicants and Drugs Analysis - LATF, Faculty of Pharmaceutical Sciences, Federal University of Alfenas - Unifal-MG, Gabriel Monteiro da Silva street, 700, 37130-000 Alfenas, MG, Brazil.
Abstract:
The development and validation of an innovative, simple, rapid, selective and sensitive vortex-assisted liquid-liquid microextraction-gas chromatography- mass spectrometry (VALLME-GC/MS) method to seven triazole fungicides in urine is reported. The confidence parameters were exhaustive evaluated and linearity (r > 0.99), precision (below 15%, to quality controls, and 20%, to lower limit of quantification), accuracy and robustness were adequate for the analysis of cyproconazole, epoxiconazole, metconazole, tebuconazole, triadimenol, propiconazole and triadimefon. To assess the applicability of the method, analyses were performed in volunteers exposed to triazoles in the last 30 days. The method demonstrated satisfactory analytical performance, with sufficient detectability to be applied as a potential tool to biomonitoring individuals exposed to the seven evaluated fungicides.
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