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Related Concept Videos

Quantitative Analysis01:12

Quantitative Analysis

1.4K
Quantitative analysis is a technique for measuring the amount of specific constituents in a sample. When the sample's composition is unknown, qualitative analysis is performed first to identify its components, which ensures that the correct substances are measured during the quantitative phase.
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the...
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Linear time-invariant Systems01:23

Linear time-invariant Systems

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A system is linear if it displays the characteristics of homogeneity and additivity, together termed the superposition property. This principle is fundamental in all linear systems. Linear time-invariant (LTI) systems include systems with linear elements and constant parameters.
The input-output behavior of an LTI system can be fully defined by its response to an impulsive excitation at its input. Once this impulse response is known, the system's reaction to any other input can be...
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¹³C NMR: ¹H–¹³C Decoupling01:04

¹³C NMR: ¹H–¹³C Decoupling

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The probability of having two carbon-13 atoms next to each other is negligible because of the low natural abundance of carbon-13. Consequently, peak splitting due to carbon-carbon spin-spin coupling is not observed in spectra. However, protons up to three sigma bonds away split the carbon signal according to the n+1 rule, resulting in complicated spectra.
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¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

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The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
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Applications Of NMR In Biology01:25

Applications Of NMR In Biology

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Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
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¹H NMR: Pople Notation01:09

¹H NMR: Pople Notation

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The Pople nomenclature system classifies spin systems based on the difference between their chemical shifts. Coupled spins are denoted by capital letters with subscripts indicating the number of equivalent nuclei. When the coupled nuclei have well-separated chemical shifts, they are assigned letters that are far apart in the alphabet, such as A and X. When the difference in chemical shifts is small, coupled nuclei are named using adjacent letters of the alphabet (AB, MN, or XY).
A proton...
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Related Experiment Video

Updated: Feb 1, 2026

Quantitative 31P NMR Analysis of Lignins and Tannins
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A field-invariant method for quantitative analysis with benchtop NMR.

Yevgen Matviychuk1, Jet Yeo1, Daniel J Holland1

  • 1University of Canterbury, Private Bag 4800, Cristchurch 8140, New Zealand.

Journal of Magnetic Resonance (San Diego, Calif. : 1997)
|December 12, 2018
PubMed
Summary
This summary is machine-generated.

A new model-based method enhances quantitative analysis for benchtop Nuclear Magnetic Resonance (NMR) spectroscopy. This approach overcomes low spectral resolution, enabling accurate composition analysis of samples like fruit juices across various field strengths.

Keywords:
Benchtop instrumentsMedium-field NMRMixture analysisQuantitative NMR spectroscopy

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Physical Chemistry

Background:

  • Benchtop Nuclear Magnetic Resonance (NMR) spectroscopy offers industrial applications but faces challenges due to low spectral resolution.
  • Traditional quantification methods struggle with the reduced spectral quality from lower field strength NMR instruments.

Purpose of the Study:

  • To develop a novel model-based quantification method for benchtop NMR spectroscopy.
  • To overcome limitations of low spectral resolution in quantitative analysis.
  • To create a method invariant to spectrometer field strength.

Main Methods:

  • A model-based approach defining quantification in terms of quantum mechanical properties of spin systems.
  • Application of the method to medium-field 1H NMR spectra.
  • Experimental validation using prepared samples and natural fruit juices.

Main Results:

  • Accurate separation of glucose anomers and monitoring of their interconversion in non-deuterated water.
  • Quantitative composition analysis of natural fruit juices using NMR data from 43 MHz to 400 MHz.
  • Good agreement between estimated compositions and reference values from nutrition databases.

Conclusions:

  • The developed model-based method is effective for quantitative analysis of benchtop 1H NMR spectra.
  • The approach provides accurate and consistent results across a range of magnetic field strengths.
  • This method broadens the applicability of NMR spectroscopy in industrial settings and for complex biological samples.