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The lungs are nestled in a cavity, shielded by the pleura. The pleura, a form of serous membrane, wraps around each lung. This membrane arrangement consists of two layers: the visceral and parietal pleurae. The visceral pleura lines the surface of the lungIn contrast, the parietal pleura is the outer layer and contacts to the thoracic wall, the mediastinum, and the diaphragm. The hilum is the point of connection between the visceral and parietal layers. The space between the parietal and...
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Related Experiment Video

Updated: Feb 1, 2026

Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells
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Transplantation of Bioengineered Lung Using Decellularized Mouse Lungs and Primary Human Endothelial Cells

Published on: March 28, 2025

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Cell replacement in human lung bioengineering.

Brandon A Guenthart1, John D O'Neill2, Jinho Kim3

  • 1Department of Surgery, Columbia University Medical Center, Columbia University, New York, New York, USA; Department of Biomedical Engineering, Columbia University Medical Center, Columbia University, New York, New York, USA.

The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation
|December 12, 2018
PubMed
Summary
This summary is machine-generated.

Researchers successfully replaced cells in donor lungs outside the body, a key step toward creating transplantable organs or new treatments for lung disease.

Keywords:
cell replacementdecellularizationex-vivo lung perfusion (EVLP)lung bioengineeringlung transplantationorgan regenerationstem cell

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Area of Science:

  • Regenerative Medicine
  • Biomedical Engineering
  • Pulmonary Research

Background:

  • Rising demand for lung transplants due to end-stage lung disease.
  • Limitations of current lung engineering and artificial devices.
  • Potential of cell-based therapeutics for lung tissue engineering.

Purpose of the Study:

  • To investigate targeted cell replacement in ex-vivo lungs.
  • To assess the feasibility of enhancing lung tissue engineering.

Main Methods:

  • Procurement and ex-vivo perfusion of human lungs unsuitable for transplantation.
  • Intratracheal delivery of decellularization solution to target lung regions.
  • Delivery and incubation of mesenchymal stem cells or airway epithelial cells.

Main Results:

  • Successful removal of airway epithelium while preserving vascular endothelium and extracellular matrix.
  • Demonstrated retention and homogeneous distribution of delivered cells within the lung.
  • Observed flattened cellular morphology of the replaced cells.

Conclusions:

  • Targeted cell replacement in extracorporeal organs is feasible.
  • Potential for developing chimeric organs for transplantation.
  • Possibility of in-situ interventions to treat or reverse lung disease, potentially reducing transplant need.