The two TRIM25 isoforms were differentially induced in Larimichthys crocea post poly (I:C) stimulation

Area of Science:

• * Molecular Biology and Immunology
• * Fish genomics and innate immune responses

Background:

• * Tripartite motif containing 25 (TRIM25) is a crucial E3 ubiquitin ligase involved in innate immunity and antiviral responses.
• * Understanding TRIM25 gene family evolution and function in teleost fish is vital for aquaculture and disease resistance.

Purpose of the Study:

• * To identify and characterize the TRIM25 gene homologue in large yellow croaker (Larimichthys crocea), named LcTRIM25.
• * To investigate the expression patterns and immune response of LcTRIM25 isoforms following viral stimulation.

Main Methods:

• * Molecular cloning and sequencing to identify LcTRIM25 isoforms.
• * Phylogenetic analysis to determine evolutionary relationships.
• * Quantitative Real-time PCR (qRT-PCR) to analyze tissue-specific expression and response to poly (I:C) stimulation.

Main Results:

• * Two LcTRIM25 isoforms, LcTRIM25-L and LcTRIM25-S, generated by alternative splicing, were identified, possessing conserved functional domains (RING, B-box2, CCD, PRY/SPRY).
• * LcTRIM25 isoforms were ubiquitously expressed in large yellow croaker tissues, with highest levels in the liver.
• * Expression of both isoforms was significantly upregulated in response to poly (I:C) stimulation, indicating a role in antiviral immunity, with differential expression patterns observed.

Conclusions:

• * LcTRIM25, with its distinct isoforms generated by alternative splicing, plays a significant role in the innate immune response of large yellow croaker.
• * The differential expression of LcTRIM25 isoforms suggests a complex regulatory mechanism and potentially dual roles in antiviral defense.
• * LcTRIM25 is a key component of the antiviral immunity in teleost fish, offering potential targets for enhancing disease resistance in aquaculture.