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Genome aware CRISPR gRNA target prediction for parasitic nematodes
1Department of Biological Sciences, George Washington University, Science and Engineering Hall, Suite 6000, 800 22nd Street NW, Washington DC 20052, USA; Institute for Neuroscience, George Washington University, 636A Ross Hall, 2300 I Street NW, Washington DC 20052, USA.
Molecular and Biochemical Parasitology
|December 12, 2018
Summary
Researchers have developed new CRISPR/Cas9 tools to study parasitic nematodes. This software identifies gene targets, aiding genetic research and overcoming challenges in genetically intractable nematode systems.
Area of Science:
- Molecular biology
- Genetics
- Parasitology
Background:
- Research into parasitic nematode molecular biology is accelerating.
- Genetic tools for studying these organisms are increasingly available.
- CRISPR/Cas9 technology presents a significant advancement for nematode research.
Purpose of the Study:
- To introduce a novel software tool for designing CRISPR/Cas9 experiments in parasitic nematodes.
- To facilitate the identification of guide RNA (gRNA) targets and diagnostic primers.
- To support research on genetically intractable nematode systems.
Main Methods:
- Development of a user-friendly online software tool.
- Utilizing user-supplied sequences for target identification.
- Interrogating parasitic nematode genomes to minimize non-specific targets.
Main Results:
- The software identifies specific gRNA targets and diagnostic primers.
- It minimizes off-target effects by analyzing nematode genomes.
- An intuitive interface aids researchers in designing CRISPR experiments.
Conclusions:
- The developed software is a valuable resource for parasitic nematode genetic research.
- It simplifies the design of CRISPR/Cas9 experiments, particularly for challenging systems.
- The tool is freely available online to the research community.
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