Graphene-oxide quenching-based molecular beacon imaging of exosome-mediated transfer of neurogenic miR-193a on
Hyun Jeong Oh1, Jaehoon Kim2, Hyejin Park2
1Department of Nuclear Medicine, Seoul National University College of Medicine, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea; Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul 110-744, Republic of Korea; School of Mechanical Engineering, Korea University, 145 Anam Dong, Seongbuk, Seoul 02841, Republic of Korea.
Abstract:
Graphene-oxide (GO) quenching-based molecular beacon was developed for rapid and sensitive detection of RNAs in living cells and tissues. Here, we applied GO quenching-based molecular beacon sensor to visualize neurogenic miR-193a levels delivered via exosomes during cell-non-autonomous neurogenesis in neural progenitor cells on a microfluidic platform. Exosomal transport was visualized using CD63-RFP plasmid vector, and FAM-labeled peptide nucleic acid (PNA) probe for the miR-193 sequence was designed to detect endogenous miR-193 expression. Fluorescence signals of FAM-PNA193a-GO were recovered in dibutyryl-cAMP-induced F11 cells, resulting from increased expression of miR-193a after neuronal differentiation. We observed delivery of miR-193a-containing exosomes released from differentiated donor F11 cells to recipient undifferentiated F11 cells. Fluorescence recovery was evident in exosome-stimulated recipient individual F11 cells in the microfluidic system. We propose molecular beacon imaging using PNA-GO complex for visualization of individual cellular expression of mature microRNAs. This system reveals the precise spatial localization and temporal sequences of mature miRNAs by intercellular exosomal delivery of messages for processes such as cell-non-autonomous neurogenesis.
More Related Videos
08:57Scalable Syntheses of Graphene Oxide and Reduced Graphene Oxide using Cascade Design Oxidation and Highly Basic Reduction Reactions
Published on: July 3, 2025
15:01Quantitative, Real-time Analysis of Base Excision Repair Activity in Cell Lysates Utilizing Lesion-specific Molecular Beacons
Published on: August 6, 2012
Related Concept Videos
03:14Oxidation Numbers
01:15Pyruvate Oxidation
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
03:11Oxidation-Reduction Reactions
02:54Molecular and Ionic Solids
Molecular Solids
Molecular crystalline solids, such as ice, sucrose (table sugar), and iodine, are solids that are composed of neutral molecules as their constituent units. These molecules are held together by weak intermolecular forces such as London dispersion forces, dipole-dipole interactions, or hydrogen bonds, which...
01:22Physiology of Respiration II: Neurogenic Control of Respiration
Central Control
The brainstem is the primary site of central control, hosting respiratory centers:
01:36Overview of Exosomes
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...