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Updated: Feb 1, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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A Single H1 Promoter Can Drive Both Guide RNA and Endonuclease Expression in the CRISPR-Cas9 System

Zongliang Gao1, Elena Herrera-Carrillo1, Ben Berkhout1

  • 1Amsterdam UMC, Department of Medical Micorbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, Amsterdam, the Netherlands.

Molecular Therapy. Nucleic Acids
|December 12, 2018
PubMed

Keywords:
CRISPR-Cas9Cas9 protein expressionH1 promoterRNA polymerase III and IIdual RNA polymerase promoterguide RNA expressionlentiviral vectorviral delivery

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