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Updated: Feb 1, 2026

Detection and Genogrouping of Noroviruses from Children's Stools By Taqman One-step RT-PCR
Published on: July 22, 2012
A semi-nested RT-PCR assay for detection of norovirus in rat fecal samples
Josélia Cristina de Oliveira Moreira1, Daniele Masselli Rodrigues1, Rovilson Gilioli1
1Cidade Universitária "Zeferino Vaz" Barão Geraldo, 250, 5 de Junho Street, Campinas - São Paulo 13083-970, Brazil.
Abstract:
Norovirus is a highly prevalent pathogen that can infect a wide range of host species. Thus far, there have only been two reports of norovirus infection in rats. Diagnostic assays for the detection of norovirus are well established, but a specific molecular assay for the diagnosis of norovirus infection in laboratory rats has not yet been reported. In this study, we describe the development of a sensitive, semi-nested RT-PCR assay for detection of norovirus in fecal samples from Rattus norvegicus, reared in animal facilities under different sanitary barrier conditions. Additionally, we describe the first report of the presence of norovirus in rat colonies from Brazilian animal facilities.
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