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Updated: Feb 1, 2026

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Published on: March 21, 2025
Riboregulated toehold-gated gRNA for programmable CRISPR-Cas9 function
1Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE, USA.
Abstract:
Predictable control over gene expression is essential to elicit desired synthetic cellular phenotypes. Although CRISPR-Cas9 offers a simple RNA-guided method for targeted transcriptional control, it lacks the ability to integrate endogenous cellular information for efficient signal processing. Here, we present a new class of riboregulators termed toehold-gated gRNA (thgRNA) by integrating toehold riboswitches into sgRNA scaffolds, and demonstrate their programmability for multiplexed regulation in Escherichia coli with minimal cross-talks.
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