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Cryopreservation of parasites.

J Eckert1

  • 1Institute of Parasitology, University of Zürich, Switzerland.

Experientia
|October 15, 1988
PubMed
Summary

Cryopreservation of helminth parasites like Echinococcus multilocularis and Toxocara canis in liquid nitrogen is effective for long-term storage. This technique preserves parasite viability and antigen quality, reducing the need for experimental animals.

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Area of Science:

  • Veterinary Parasitology
  • Cryobiology
  • Molecular Biology

Background:

  • Cryopreservation offers a method for long-term storage of parasite isolates.
  • Maintaining parasite viability is crucial for research and diagnostics.
  • Reducing the use of experimental animals is an ethical and practical consideration in parasitology.

Purpose of the Study:

  • To review advances in cryopreservation techniques for helminth parasites.
  • To evaluate the viability and immunodiagnostic quality of cryopreserved helminth parasites.
  • To assess the feasibility of cryopreservation for various helminth species.

Main Methods:

  • Deep-freezing and storage in liquid nitrogen for Echinococcus multilocularis metacestodes.
  • Cryopreservation of Toxocara canis infective larvae in liquid nitrogen.
  • In vitro culture of cryopreserved larvae in a chemically defined medium.
  • Assessment of larval motility and secretory/excretory antigen quality post-thawing.

Main Results:

  • Echinococcus multilocularis metacestodes remained viable for 1-2 years after cryopreservation.
  • Toxocara canis larvae survived cryopreservation for 35 weeks in vitro, retaining immunodiagnostic antigen quality despite reduced motility.
  • Cryopreservation success varied, with some species like Dictyocaulus viviparus and Trichinella spiralis showing sensitivity to subzero temperatures.

Conclusions:

  • Cryopreservation is a valuable tool for the long-term storage of defined helminth parasite isolates.
  • The technique can significantly reduce the number of experimental animals required for serial passages.
  • Further optimization of cooling protocols may improve cryopreservation success rates for sensitive parasite species.

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