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Cloning a synthetic gene for human stefin B and its expression in E. coli
R Jerala1, M Trstenjak, B Lenarcic
1Department of Biochemistry, Jozef Stefan Institute, Ljubljana, Yugoslavia.
FEBS Letters
|October 24, 1988
Abstract:
A gene coding for human stefin B was synthesized by the solid-phase phosphite method and cloned in the pUC8 cloning vector. The insert with the verified DNA sequence was subcloned into two expression vectors and expressed in E. coli as a fusion protein with beta-galactosidase and as a native protein. The CNBr cleaved fusion protein and the native recombinant stefin B were inhibitory to papain and reacted with antibodies against human stefin B.