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The relationship between bacterial sources and genotype to the antimicrobial resistance pattern of Burkholderia

Muhammad Abubakar Sadiq1,2, Latiffah Hassan1, Saleha Abdul Aziz1

  • 1Department of Microbiology and Pathology, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), 44300 UPM Serdang, Selangor Darul Ehsan Malaysia.

Veterinary World
|December 12, 2018
PubMed
Abstract

Insights

Antibiotic resistance in Burkholderia pseudomallei varies by isolate source and genotype. Veterinary isolates and novel ST 1130 strains showed higher resistance to meropenem (MEM) and cotrimoxazole (SXT).

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Melioidosis, a fatal emerging infectious disease, is caused by Burkholderia pseudomallei.
  • Potential variations in B. pseudomallei strains may influence antibiotic susceptibility and resistance patterns.

Purpose of the Study:

  • To investigate the association between the phenotypic antibiotic resistance patterns of B. pseudomallei.
  • To determine if resistance patterns correlate with isolate source (veterinary vs. environmental) and bacterial genotype.

Main Methods:

  • Phylogenetic characterization of 111 B. pseudomallei isolates using multilocus sequence typing (MLST).
  • Antimicrobial susceptibility testing (minimum inhibitory concentration) for meropenem (MEM), imipenem, ceftazidime (CAZ), cotrimoxazole (SXT), and co-amoxiclav (AMC).

Main Results:

  • Most isolates were susceptible to tested antibiotics; however, resistance to MEM, SXT, CAZ, and AMC was observed in a few strains.
  • A statistically significant association was found between resistance to MEM and veterinary clinical isolates (p<0.05).
  • Novel ST 1130 isolates from veterinary cases exhibited a significantly higher likelihood of MEM resistance compared to other isolates.

Conclusions:

  • Resistance to MEM and SXT appears more prevalent in veterinary B. pseudomallei isolates.
  • The novel ST 1130 genotype is associated with an increased likelihood of MEM resistance.
Keywords:
Burkholderia pseudomalleianimalsantimicrobialenvironmentalresistancesequence typesveterinary isolates

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