Related Experiment Video

Updated: Feb 1, 2026

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance
08:45

Whole Genome Sequencing of Candida glabrata for Detection of Markers of Antifungal Drug Resistance

Published on: December 28, 2017

14.2K

Draft Genome Sequence of the Extensively Drug-Resistant Pseudomonas aeruginosa Clinical Isolate TUEPA7472

Henrike Miess1, Ghazaleh Jahanshah1,2, Heike Brötz-Oesterhelt2,3

  • 1Department of Pharmaceutical Biology, Institute of Pharmaceutical Sciences, University of Tübingen, Tübingen, Germany.

Microbiology Resource Announcements
|December 12, 2018
PubMed

Insights

This study details the genome of extensively drug-resistant Pseudomonas aeruginosa TUEPA7472. The findings reveal mechanisms behind its multiresistance to four antibiotic classes and potential virulence factors.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Pseudomonas aeruginosa is a significant opportunistic pathogen.
  • Extensively drug-resistant (XDR) strains pose a major public health threat.
  • Multiresistance toward 4 classes of clinically relevant antibiotics (4MRGN) complicates treatment options.

Purpose of the Study:

  • To perform a draft genome sequencing of the XDR Pseudomonas aeruginosa TUEPA7472 strain.
  • To investigate the genetic basis of its antibiotic resistance mechanisms.
  • To identify potential virulence factors encoded in the genome.

Main Methods:

  • Whole-genome sequencing of Pseudomonas aeruginosa TUEPA7472.
  • Bioinformatic analysis of the draft genome sequence.
  • Identification of genes associated with antibiotic resistance and virulence.

Main Results:

  • The 6.8-Mb draft genome sequence of Pseudomonas aeruginosa TUEPA7472 was obtained.
  • Analysis provided insights into the genetic determinants of its extensive drug resistance.
  • Potential virulence factors were identified within the genome.

Conclusions:

  • The genome sequence offers a valuable resource for understanding XDR Pseudomonas aeruginosa.
  • Insights into resistance mechanisms can inform the development of new therapeutic strategies.
  • Further research into identified virulence factors may elucidate pathogenicity.

More Related Videos

Modeling Persistent Pseudomonas aeruginosa Infection in Wounded Zebrafish Larvae06:04

Modeling Persistent Pseudomonas aeruginosa Infection in Wounded Zebrafish Larvae

Published on: June 13, 2025

671
Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control05:09

Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control

Published on: March 15, 2024

6.0K

Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.6K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
Clinically Relevant Drug Product Specifications: Methods of Establishment01:29

Clinically Relevant Drug Product Specifications: Methods of Establishment

Product specifications define the acceptable quality of a pharmaceutical product by ensuring identity, purity, potency, and strength. These specifications serve as benchmarks during development, manufacturing, and post-approval quality control. Clinically relevant specifications are particularly important because they directly relate to a drug's safety and efficacy in clinical use.Dissolution studies are critical biopharmaceutic tools that link in vitro behavior to in vivo performance. They...
217
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.0K