Related Experiment Video

Updated: Feb 1, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

22.3K

Draft Genome Sequences of Two Multidrug-Resistant Salmonella enterica Serovar Typhimurium Clinical Isolates from

Nicolás F Cordeiro1, Bruno D'Alessandro2, Andrés Iriarte2

  • 1Departamento de Bacteriología y Virología, Instituto de Higiene, Facultad de Medicina, UDELAR, Montevideo, Uruguay.

Microbiology Resource Announcements
|December 12, 2018
PubMed

Insights

Multidrug-resistant Salmonella enterica strains pose a global health threat. This study defines the genomic basis of antimicrobial resistance in two specific Salmonella Typhimurium isolates using next-generation sequencing.

Area of Science:

  • Microbiology
  • Genomics
  • Infectious Diseases

Background:

  • Multidrug-resistant Salmonella enterica is a growing global health concern.
  • The genetic mechanisms underlying this resistance are often poorly understood.
  • Salmonella Typhimurium is a significant pathogen responsible for numerous infections.

Purpose of the Study:

  • To investigate the genomic characteristics of multidrug-resistant Salmonella Typhimurium isolates.
  • To identify specific antimicrobial resistance genes within these isolates.
  • To elucidate the genetic basis of multidrug resistance in clinical Salmonella strains.

Main Methods:

  • Whole-genome sequencing of two multidrug-resistant Salmonella Typhimurium isolates (ST3224 and ST827).
  • Bioinformatic analysis to identify antimicrobial resistance genes and genomic features.
  • Comparative genomics to understand resistance mechanisms.

Main Results:

  • Detailed genomic profiles of the two Salmonella Typhimurium isolates were established.
  • Specific antimicrobial resistance genes conferring resistance to multiple drug classes were identified.
  • Genomic data provided insights into the evolution and dissemination of resistance.

Conclusions:

  • Next-generation sequencing is crucial for defining antimicrobial resistance mechanisms in Salmonella.
  • Understanding the genomic basis of resistance is essential for effective treatment and control strategies.
  • This study contributes to the knowledge of multidrug resistance in Salmonella Typhimurium.

More Related Videos

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages10:33

Isolation of Salmonella typhimurium-containing Phagosomes from Macrophages

Published on: October 25, 2017

11.2K
High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages11:10

High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages

Published on: August 11, 2014

13.2K

Related Concept Videos

Genomics02:02

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.6K
Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
16.0K
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K