Related Experiment Video
Updated: Feb 1, 2026
04:41
Plaquing of Herpes Simplex Viruses
Published on: November 5, 2021
7.1K
Complete Genome Sequence of Herpes Simplex Virus 2 Strain 333
Alberto Domingo López-Muñoz1, Alberto Rastrojo1, Antonio Alcamí1
1Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Científicas and Universidad Autónoma de Madrid), Madrid, Spain.
Microbiology Resource Announcements
|December 12, 2018
Summary
Herpes simplex virus 2 (human herpesvirus 2) causes genital sores and latency. This study fully sequenced and curated the genome of HSV-2 strain 333 for further research.
Area of Science:
- Virology
- Genomics
- Infectious Diseases
Background:
- Herpes simplex virus 2 (human herpesvirus 2) is a widespread human pathogen.
- HSV-2 is known to cause genital ulcerations.
- The virus establishes latency in sacral root ganglia.
Purpose of the Study:
- To perform a complete sequencing of the herpes simplex virus 2 genome.
- To manually curate the obtained viral genome sequence of HSV-2 strain 333.
Main Methods:
- Utilized Pacific Biosciences sequencing technology.
- Employed Illumina sequencing technology for genome analysis.
- Manual curation of the viral genome sequence.
Main Results:
- Successfully obtained the full genome sequence of herpes simplex virus 2, strain 333.
- The genome sequence was manually curated for accuracy and completeness.
Conclusions:
- The fully sequenced and curated HSV-2 strain 333 genome provides a valuable resource.
- This genomic data can facilitate further research into HSV-2 pathogenesis and latency.
Related Concept Videos
01:29Viruses with RNA Genomes
903
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
903
02:02Genomics
40.6K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
40.6K
00:50What are Viruses?
128.1K
Overview
128.1K
02:30Genomic Imprinting and Inheritance
37.2K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
37.2K
03:21Genome Size and the Evolution of New Genes
9.1K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.1K
02:02Cis-regulatory Sequences
11.8K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K