Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

9.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.4K
Preparation of 1° Amines: Azide Synthesis01:22

Preparation of 1° Amines: Azide Synthesis

4.6K
Direct alkylation of ammonia produces polyalkylated amines, along with a quaternary ammonium salt. To exclusively prepare primary amines, the azide synthesis method can be used.
Azide ions act as good nucleophiles and react with unhindered alkyl halides to form alkyl azides. Alkyl azides do not participate in further nucleophilic substitution reactions, thereby eliminating the chances of polyalkylated products. Alkyl azides are reduced by hydride-based reducing agents, like lithium aluminum...
4.6K
Labeling Emotion01:20

Labeling Emotion

719
Emotional labeling is a cognitive process that involves identifying and naming one's emotions, such as anger, fear, happiness, or sadness. It allows individuals to recognize and express their internal emotional states, a critical aspect of emotional regulation and communication. Labeling emotions requires more than mere recognition; it also involves drawing upon memory and contextual cues to understand the current situation and apply a corresponding emotional label. For instance, feeling...
719
Lewis Acids and Bases02:33

Lewis Acids and Bases

48.4K
In 1923, G. N. Lewis proposed a generalized definition of acid-base behavior in which acids and bases are identified by their ability to accept or to donate a pair of electrons and form a coordinate covalent bond.
A coordinate covalent bond (or dative bond) occurs when one of the atoms in the bond provides both bonding electrons. For example, a coordinate covalent bond occurs when a water molecule combines with a hydrogen ion to form a hydronium ion. A coordinate covalent bond also results when...
48.4K
Weak Base Solutions03:21

Weak Base Solutions

25.2K
Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
25.2K
Ions as Acids and Bases02:54

Ions as Acids and Bases

26.4K
Salts with Acidic Ions
Salts are ionic compounds composed of cations and anions, either of which may be capable of undergoing an acid or base ionization reaction with water. Aqueous salt solutions, therefore, may be acidic, basic, or neutral, depending on the relative acid-base strengths of the salt’s constituent ions. For example, dissolving the ammonium chloride in water results in its dissociation, as described by the equation:
26.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Follicular Lymphoma Developing During IL-17A Inhibitor Therapy in a Patient With Psoriasis: Retreatment Without Lymphoma Progression.

International journal of dermatology·2026
Same author

Quinoline-Directed Folding in Anilide-Quinoline Hybrids: Length-Controlled Three-Dimensional Conformational Diversity.

JACS Au·2026
Same author

Catalytic Asymmetric Addition of Acetonitrile to Aryl Alkyl Ketimines for the Construction of Tetrasubstituted Stereogenic Centers.

Organic letters·2026
Same author

Skeletal Modification of Phenols into Benzo-Fused Seven-Membered N,O-Heterocycles via Catalytic Alkyl Nitrene C(sp<sup>2</sup>)-H Amination.

Organic letters·2026
Same author

Revisiting C4N4 Fluorophore: Theoretical Elucidation of the Origin of Fluorescent Properties of 2,5-Diaminopyrimidines and Strategic Applications to Pd Detection.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026
Same author

Catalytic Asymmetric Benzoin Condensation for the Synthesis of Natural Products and Their Analogs.

Organic letters·2025

Related Experiment Video

Updated: Feb 1, 2026

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

22.3K

A fluorogenic C4N4 probe for azide-based labelling.

Hidetoshi Noda1, Yasuko Asada, Masakatsu Shibasaki

  • 1Institute of Microbial Chemistry (BIKAKEN), 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141-0021, Japan. mshibasa@bikaken.or.jp nkumagai@bikaken.or.jp.

Organic & Biomolecular Chemistry
|December 12, 2018
PubMed
Summary

A novel fluorogenic probe was synthesized using the 2,5-diaminopyrimidine fluorophore. This probe shows a significant 30-fold increase in fluorescence upon reaction, making it useful for detection applications.

More Related Videos

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
07:23

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Published on: October 10, 2016

8.6K
Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
09:31

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Published on: September 17, 2021

4.8K

Related Experiment Videos

Last Updated: Feb 1, 2026

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
09:06

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition

Published on: December 23, 2016

22.3K
An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
07:23

An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group

Published on: October 10, 2016

8.6K
Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe
09:31

Analyzing Oxidative Stress in Murine Intestinal Organoids using Reactive Oxygen Species-Sensitive Fluorogenic Probe

Published on: September 17, 2021

4.8K

Area of Science:

  • Organic chemistry
  • Chemical biology
  • Materials science

Background:

  • Development of sensitive fluorescent probes is crucial for various analytical and biological applications.
  • The 2,5-diaminopyrimidine (C4N4) moiety is a recently identified fluorophore with potential for probe development.

Purpose of the Study:

  • To design, synthesize, and characterize a new fluorogenic probe based on the C4N4 fluorophore.
  • To evaluate the photophysical properties and fluorescence enhancement of the synthesized probe.

Main Methods:

  • Design and synthesis of an azido-functionalized C4N4 probe.
  • Strain-promoted [3 + 2] cycloaddition reaction with a suitable dienophile.
  • Spectroscopic analysis to determine fluorescence intensity and enhancement.

Main Results:

  • Successful synthesis of the azido-C4N4 probe.
  • Formation of a triazole product via cycloaddition.
  • Demonstrated over 30-fold fluorescence enhancement in the triazole compared to the azide precursor.

Conclusions:

  • The new C4N4-based fluorogenic probe exhibits significant fluorescence enhancement upon cycloaddition.
  • This probe holds promise for sensitive detection and imaging applications.