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Morphological features predictive for BRAF(V600E) mutation in papillary thyroid microcarcinomas.

Adela Corina Nechifor-Boilă1, Emőke Andrea Szász, Françoise Descotes

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Summary
This summary is machine-generated.

Histological features like subcapsular localization and stromal reaction can predict the BRAF V600E mutation in papillary thyroid microcarcinomas (PTMCs). This aids in risk stratification and guiding adjuvant therapy decisions.

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Area of Science:

  • Oncology
  • Pathology
  • Genetics

Background:

  • The BRAF V600E mutation is a specific marker for papillary thyroid carcinoma (PTC), including microcarcinomas (PTMCs).
  • Assessing BRAF V600E mutational status is costly and not universally available.
  • Identifying predictive morphological features can improve diagnostic accessibility.

Purpose of the Study:

  • To identify morphological features that predict the BRAF V600E mutation in PTMCs.
  • To evaluate the utility of routine histopathology in inferring molecular status.
  • To potentially reduce reliance on expensive genetic testing.

Main Methods:

  • Analysis of 19 PTMCs (25 foci) for histological features: size, multifocality, extrathyroidal extension, border, nuclear features, stromal reaction, and variant.
  • BRAF V600E mutation detection using RT-PCR, high-resolution melting (HRM) analysis, and Sanger sequencing.
  • Comparison of morphological features between BRAF V600E positive and negative PTMCs using Fisher's exact test.

Main Results:

  • BRAF V600E mutation was present in 36% of PTMC foci.
  • Subcapsular localization (p=0.013), conventional histological type (p=0.05), and moderate/extensive stromal reaction (p=0.032) were significantly associated with the BRAF V600E mutation.

Conclusions:

  • Morphological features can reliably predict BRAF V600E mutation status in PTMCs.
  • Documenting these features in histopathological reports can aid risk stratification.
  • This approach may help select patients requiring adjuvant post-surgery therapy.