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Long non‑coding RNA DANCR promotes HMGA2‑mediated invasion in lung adenocarcinoma cells

Ningning Zhang1, Wei Jiang2

  • 1Youth League Committee, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China.

Oncology Reports
|December 12, 2018
PubMed

Insights

Long non-coding RNA DANCR promotes lung adenocarcinoma invasion by upregulating HMGA2. This DANCR/HMGA2 pathway presents a potential new target for treating lung adenocarcinoma.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are implicated in cancer development.
  • The specific roles of lncRNA DANCR and HMGA2 in lung adenocarcinoma (LAD) are not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of lncRNA DANCR and HMGA2 in lung adenocarcinoma.
  • To explore the potential of the DANCR/HMGA2 axis as a therapeutic target for LAD.

Main Methods:

  • Quantitative real-time PCR to measure DANCR and HMGA2 expression in LAD tissues and cell lines.
  • Cell invasion assays (e.g., Transwell assay) to assess the effect of DANCR and HMGA2.
  • RNA interference (siRNA) to knockdown DANCR and HMGA2 expression in LAD cells.

Main Results:

  • DANCR expression was significantly upregulated in LAD tissues and cell lines compared to normal controls.
  • Elevated DANCR expression correlated with poor prognosis in LAD patients.
  • Knockdown of DANCR inhibited LAD cell invasion and reduced HMGA2 expression.
  • HMGA2 was overexpressed in LAD and its inhibition suppressed cell invasion.
  • DANCR promoted LAD cell invasion by positively regulating HMGA2 expression.

Conclusions:

  • The lncRNA DANCR promotes lung adenocarcinoma cell invasion through the upregulation of HMGA2.
  • The DANCR/HMGA2 axis represents a promising novel therapeutic target for lung adenocarcinoma treatment.

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