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Related Experiment Video

Updated: Feb 1, 2026

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Published on: February 6, 2015

The Elevated Plus Maze Test for Measuring Anxiety-Like Behavior in Rodents.

Ann-Katrin Kraeuter^1,2, Paul C Guest³, Zoltán Sarnyai^4,5

¹Laboratory of Psychiatric Neuroscience, Australian Institute of Tropical Health and Medicine, James Cook University, Townsville, QLD, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|December 12, 2018

Summary

The elevated plus maze test assesses anxiety in rodents by observing their aversion to open spaces. This method aids in understanding anxiety disorders and screening potential anxiety-reducing drugs.

Keywords:

Anxiety Anxiolytic Behavior Elevated plus maze Mouse Rodent

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Last Updated: Feb 1, 2026

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Use of the Open Field Maze to Measure Locomotor and Anxiety-like Behavior in Mice

Published on: February 6, 2015

Testing Animal Anxiety in Rats: Effects of Open Arm Ledges and Closed Arm Wall Transparency in Elevated Plus Maze Test

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Published on: June 29, 2018

The Double-H Maze: A Robust Behavioral Test for Learning and Memory in Rodents

The Double-H Maze: A Robust Behavioral Test for Learning and Memory in Rodents

Published on: July 8, 2015

Area of Science:

Behavioral Neuroscience
Pharmacology
Animal Models

Background:

Anxiety-like behavior is a key feature in conditions like post-traumatic stress disorder (PTSD).
Rodent models are crucial for studying anxiety and testing therapeutic interventions.
The elevated plus maze (EPM) is a widely used behavioral test for anxiety assessment.

Purpose of the Study:

To detail the methodology for conducting the elevated plus maze test in rodents.
To provide guidance on interpreting EPM test results for anxiety-like behavior.
To highlight the utility of the EPM in preclinical drug screening for anxiolytic compounds.

Main Methods:

The EPM test is based on the innate aversion rodents exhibit towards open, elevated spaces.
Behavioral data is collected by measuring time spent in and entries into the open and closed arms of the maze.
Detailed protocols for test setup, animal handling, and data acquisition are described.

Main Results:

Increased time spent in the enclosed arms and fewer entries into open arms indicate higher anxiety-like behavior.
Variations in these parameters can reflect the impact of experimental conditions or pharmacological treatments.
The test provides quantifiable measures of anxiety-related responses in rodents.

Conclusions:

The elevated plus maze is a valuable tool for quantifying anxiety-like behavior in rodents.
It serves as a critical model for investigating the neurobiology of anxiety disorders.
The EPM facilitates the screening and development of novel anxiolytic agents.